Nanoscale ion sequestration to determine the polarity selectivity of ion conductance in carriers and channels

Cranfield, CG; Bettler, T; Cornell, B

Nanoscale ion sequestration to determine the polarity selectivity of ion conductance in carriers and channels

Cranfield, CG

Bettler, T Cornell, B

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Publication Type:: Journal Article
Citation:: Langmuir, 2015, 31 (1), pp. 292 - 298
Issue Date:: 2015-01-13

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Field	Value	Language
dc.contributor.author	Cranfield, CG https://orcid.org/0000-0003-3608-5440	en_US
dc.contributor.author	Bettler, T	en_US
dc.contributor.author	Cornell, B https://orcid.org/0000-0003-4166-3241	en_US
dc.date.issued	2015-01-13	en_US
dc.identifier.citation	Langmuir, 2015, 31 (1), pp. 292 - 298	en_US
dc.identifier.issn	0743-7463	en_US
dc.identifier.uri	http://hdl.handle.net/10453/31119
dc.description.abstract	© 2014 American Chemical Society. The nanoscale spacing between a tethered lipid bilayer membrane (tBLM) and its supporting gold electrode can be utilized to determine the polarity selectivity of the conduction of ion channels and ion carriers embedded in a membrane. The technique relies upon a bias voltage sequestering or eliminating ions, of a particular polarity, into or out of the aqueous electrolyte region between the gold electrode and the tethered membrane. A demonstration is given, using ac swept frequency impedance spectrometry, of the bias polarity dependence of the ionophore conductance of gramicidin A, a cationic selective channel, and valinomycin, a potassium ion selective carrier. We further use pulsed amperometry to show that the intrinsic voltage dependence of the ion conduction is actually selective of the polarity of the transported ion and not simply of the direction of the ionic current flow.	en_US
dc.relation.ispartof	Langmuir	en_US
dc.relation.isbasedon	10.1021/la504057z	en_US
dc.subject.classification	Chemical Physics	en_US
dc.subject.mesh	Ions	en_US
dc.subject.mesh	Valinomycin	en_US
dc.subject.mesh	Gramicidin	en_US
dc.subject.mesh	Electric Conductivity	en_US
dc.subject.mesh	Nanotechnology	en_US
dc.subject.mesh	Models, Biological	en_US
dc.title	Nanoscale ion sequestration to determine the polarity selectivity of ion conductance in carriers and channels	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	31	en_US
utslib.for	1007 Nanotechnology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	31	en_US

Abstract:

© 2014 American Chemical Society. The nanoscale spacing between a tethered lipid bilayer membrane (tBLM) and its supporting gold electrode can be utilized to determine the polarity selectivity of the conduction of ion channels and ion carriers embedded in a membrane. The technique relies upon a bias voltage sequestering or eliminating ions, of a particular polarity, into or out of the aqueous electrolyte region between the gold electrode and the tethered membrane. A demonstration is given, using ac swept frequency impedance spectrometry, of the bias polarity dependence of the ionophore conductance of gramicidin A, a cationic selective channel, and valinomycin, a potassium ion selective carrier. We further use pulsed amperometry to show that the intrinsic voltage dependence of the ion conduction is actually selective of the polarity of the transported ion and not simply of the direction of the ionic current flow.

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http://hdl.handle.net/10453/31119