Structure and function of a spectrin-like regulator of bacterial cytokinesis

Cleverley, RM; Barrett, JR; Baslé, A; Bui, NK; Hewitt, L; Solovyova, A; Xu, ZQ; Daniel, RA; Dixon, NE; Harry, EJ; Oakley, AJ; Vollmer, W; Lewis, RJ

Structure and function of a spectrin-like regulator of bacterial cytokinesis

Cleverley, RM Barrett, JR Baslé, A Bui, NK Hewitt, L Solovyova, A Xu, ZQ Daniel, RA Dixon, NE Harry, EJ

Oakley, AJ Vollmer, W Lewis, RJ

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Publication Type:: Journal Article
Citation:: Nature Communications, 2014, 5
Issue Date:: 2014-01-01

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Field	Value	Language
dc.contributor.author	Cleverley, RM	en_US
dc.contributor.author	Barrett, JR	en_US
dc.contributor.author	Baslé, A	en_US
dc.contributor.author	Bui, NK	en_US
dc.contributor.author	Hewitt, L	en_US
dc.contributor.author	Solovyova, A	en_US
dc.contributor.author	Xu, ZQ	en_US
dc.contributor.author	Daniel, RA	en_US
dc.contributor.author	Dixon, NE	en_US
dc.contributor.author	Harry, EJ https://orcid.org/0000-0003-0858-9473	en_US
dc.contributor.author	Oakley, AJ	en_US
dc.contributor.author	Vollmer, W	en_US
dc.contributor.author	Lewis, RJ	en_US
dc.date.available	2014-09-30	en_US
dc.date.issued	2014-01-01	en_US
dc.identifier.citation	Nature Communications, 2014, 5	en_US
dc.identifier.uri	http://hdl.handle.net/10453/32289
dc.description.abstract	© 2014 Macmillan Publishers Limited. All rights reserved. Bacterial cell division is facilitated by a molecular machine - the divisome - that assembles at mid-cell in dividing cells. The formation of the cytokinetic Z-ring by the tubulin homologue FtsZ is regulated by several factors, including the divisome component EzrA. Here we describe the structure of the 60-kDa cytoplasmic domain of EzrA, which comprises five linear repeats of an unusual triple helical bundle. The EzrA structure is bent into a semicircle, providing the protein with the potential to interact at both N- and C-termini with adjacent membrane-bound divisome components. We also identify at least two binding sites for FtsZ on EzrA and map regions of EzrA that are responsible for regulating FtsZ assembly. The individual repeats, and their linear organization, are homologous to the spectrin proteins that connect actin filaments to the membrane in eukaryotes, and we thus propose that EzrA is the founding member of the bacterial spectrin family.	en_US
dc.relation.ispartof	Nature Communications	en_US
dc.relation.isbasedon	10.1038/ncomms6421	en_US
dc.subject.mesh	Bacillus subtilis	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Spectrin	en_US
dc.subject.mesh	Cytoskeletal Proteins	en_US
dc.subject.mesh	Crystallography, X-Ray	en_US
dc.subject.mesh	Cytokinesis	en_US
dc.subject.mesh	Binding Sites	en_US
dc.title	Structure and function of a spectrin-like regulator of bacterial cytokinesis	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

© 2014 Macmillan Publishers Limited. All rights reserved. Bacterial cell division is facilitated by a molecular machine - the divisome - that assembles at mid-cell in dividing cells. The formation of the cytokinetic Z-ring by the tubulin homologue FtsZ is regulated by several factors, including the divisome component EzrA. Here we describe the structure of the 60-kDa cytoplasmic domain of EzrA, which comprises five linear repeats of an unusual triple helical bundle. The EzrA structure is bent into a semicircle, providing the protein with the potential to interact at both N- and C-termini with adjacent membrane-bound divisome components. We also identify at least two binding sites for FtsZ on EzrA and map regions of EzrA that are responsible for regulating FtsZ assembly. The individual repeats, and their linear organization, are homologous to the spectrin proteins that connect actin filaments to the membrane in eukaryotes, and we thus propose that EzrA is the founding member of the bacterial spectrin family.

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http://hdl.handle.net/10453/32289