Direct measurement of dimethylsulfoniopropionate (DMSP) in reef-building corals using quantitative nuclear magnetic resonance (qNMR) spectroscopy

Tapiolas, DM; Raina, JB; Lutz, A; Willis, BL; Motti, CA

Direct measurement of dimethylsulfoniopropionate (DMSP) in reef-building corals using quantitative nuclear magnetic resonance (qNMR) spectroscopy

Tapiolas, DM Raina, JB

Lutz, A Willis, BL Motti, CA

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Publication Type:: Journal Article
Citation:: Journal of Experimental Marine Biology and Ecology, 2013, 443 pp. 85 - 89
Issue Date:: 2013-05-01

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Field	Value	Language
dc.contributor.author	Tapiolas, DM	en_US
dc.contributor.author	Raina, JB https://orcid.org/0000-0002-7508-0004	en_US
dc.contributor.author	Lutz, A	en_US
dc.contributor.author	Willis, BL	en_US
dc.contributor.author	Motti, CA	en_US
dc.date.issued	2013-05-01	en_US
dc.identifier.citation	Journal of Experimental Marine Biology and Ecology, 2013, 443 pp. 85 - 89	en_US
dc.identifier.issn	0022-0981	en_US
dc.identifier.uri	http://hdl.handle.net/10453/32382
dc.description.abstract	Reef building corals are among the largest producers of dimethylsulfoniopropionate (DMSP), a sulfur molecule synthesized by their endosymbiotic dinoflagellates in the genus Symbiodinium. DMSP is potentially involved in important physiological and ecological processes in corals, but investigating the functional role of this molecule requires rapid and accurate quantification techniques. Here we introduce a simple method enabling direct quantification of DMSP and one of its breakdown products acrylate using quantitative nuclear magnetic resonance (qNMR) spectroscopy. The method was tested on a range of coral genera and presents a number of advantages over currently used quantification techniques, including simultaneous and direct quantification of multiple molecules from the same extract, and rapid processing with high reproducibility enabling analyses of large numbers of samples in short time periods. The method was successfully applied to environmental samples and provides the first baseline information on diel variation of DMSP and acrylate concentrations in the coral Acropora millepora. •We describe a new method enabling direct quantification of DMSP in corals.•This method presents several advantages over current measurement techniques.•It was successfully applied to measure diurnal DMSP variations in corals.•The method could easily be used to quantify DMSP in other organisms. © 2013 Elsevier B.V..	en_US
dc.relation.ispartof	Journal of Experimental Marine Biology and Ecology	en_US
dc.relation.isbasedon	10.1016/j.jembe.2013.02.037	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.title	Direct measurement of dimethylsulfoniopropionate (DMSP) in reef-building corals using quantitative nuclear magnetic resonance (qNMR) spectroscopy	en_US
dc.type	Journal Article
utslib.citation.volume	443	en_US
utslib.for	0501 Ecological Applications	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	443	en_US

Abstract:

Reef building corals are among the largest producers of dimethylsulfoniopropionate (DMSP), a sulfur molecule synthesized by their endosymbiotic dinoflagellates in the genus Symbiodinium. DMSP is potentially involved in important physiological and ecological processes in corals, but investigating the functional role of this molecule requires rapid and accurate quantification techniques. Here we introduce a simple method enabling direct quantification of DMSP and one of its breakdown products acrylate using quantitative nuclear magnetic resonance (qNMR) spectroscopy. The method was tested on a range of coral genera and presents a number of advantages over currently used quantification techniques, including simultaneous and direct quantification of multiple molecules from the same extract, and rapid processing with high reproducibility enabling analyses of large numbers of samples in short time periods. The method was successfully applied to environmental samples and provides the first baseline information on diel variation of DMSP and acrylate concentrations in the coral Acropora millepora. •We describe a new method enabling direct quantification of DMSP in corals.•This method presents several advantages over current measurement techniques.•It was successfully applied to measure diurnal DMSP variations in corals.•The method could easily be used to quantify DMSP in other organisms. © 2013 Elsevier B.V..

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http://hdl.handle.net/10453/32382