The sub-cellular localization of Sulfolobus DNA replication

Gristwood, T; Duggin, IG; Wagner, M; Albers, SV; Bell, SD

The sub-cellular localization of Sulfolobus DNA replication

Gristwood, T Duggin, IG

Wagner, M Albers, SV Bell, SD

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Publication Type:: Journal Article
Citation:: Nucleic Acids Research, 2012, 40 (12), pp. 5487 - 5496
Issue Date:: 2012-07-01

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Field	Value	Language
dc.contributor.author	Gristwood, T	en_US
dc.contributor.author	Duggin, IG https://orcid.org/0000-0003-4868-7350	en_US
dc.contributor.author	Wagner, M	en_US
dc.contributor.author	Albers, SV	en_US
dc.contributor.author	Bell, SD	en_US
dc.date.issued	2012-07-01	en_US
dc.identifier.citation	Nucleic Acids Research, 2012, 40 (12), pp. 5487 - 5496	en_US
dc.identifier.issn	0305-1048	en_US
dc.identifier.uri	http://hdl.handle.net/10453/32401
dc.description.abstract	Analyses of the DNA replication-associated proteins of hyperthermophilic archaea have yielded considerable insight into the structure and biochemical function of these evolutionarily conserved factors. However, little is known about the regulation and progression of DNA replication in the context of archaeal cells. In the current work, we describe the generation of strains of Sulfolobus solfataricus and Sulfolobus acidocaldarius that allow the incorporation of nucleoside analogues during DNA replication. We employ this technology, in conjunction with immunolocalization analyses of replisomes, to investigate the sub-cellular localization of nascent DNA and replisomes. Our data reveal a peripheral localization of replisomes in the cell. Furthermore, while the two replication forks emerging from any one of the three replication origins in the Sulfolobus chromosome remain in close proximity, the three origin loci are separated. © The Author(s) 2012. Published by Oxford University Press.	en_US
dc.relation.ispartof	Nucleic Acids Research	en_US
dc.relation.isbasedon	10.1093/nar/gks217	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Sulfolobus acidocaldarius	en_US
dc.subject.mesh	Sulfolobus solfataricus	en_US
dc.subject.mesh	Multienzyme Complexes	en_US
dc.subject.mesh	DNA-Directed DNA Polymerase	en_US
dc.subject.mesh	DNA, Archaeal	en_US
dc.subject.mesh	Deoxyuridine	en_US
dc.subject.mesh	Bromodeoxyuridine	en_US
dc.subject.mesh	Cell Cycle	en_US
dc.subject.mesh	DNA Replication	en_US
dc.title	The sub-cellular localization of Sulfolobus DNA replication	en_US
dc.type	Journal Article
utslib.citation.volume	12	en_US
utslib.citation.volume	40	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0604 Genetics	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	08 Information and Computing Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	12	en_US
pubs.publication-status	Published	en_US
pubs.volume	40	en_US

Abstract:

Analyses of the DNA replication-associated proteins of hyperthermophilic archaea have yielded considerable insight into the structure and biochemical function of these evolutionarily conserved factors. However, little is known about the regulation and progression of DNA replication in the context of archaeal cells. In the current work, we describe the generation of strains of Sulfolobus solfataricus and Sulfolobus acidocaldarius that allow the incorporation of nucleoside analogues during DNA replication. We employ this technology, in conjunction with immunolocalization analyses of replisomes, to investigate the sub-cellular localization of nascent DNA and replisomes. Our data reveal a peripheral localization of replisomes in the cell. Furthermore, while the two replication forks emerging from any one of the three replication origins in the Sulfolobus chromosome remain in close proximity, the three origin loci are separated. © The Author(s) 2012. Published by Oxford University Press.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/32401