New strategies to measure intracellular sodium concentrations

Dietrich, S; Stanca, SE; Cranfield, CG; Hoffmann, B; Benndorf, K; Biskup, C

New strategies to measure intracellular sodium concentrations

Dietrich, S Stanca, SE Cranfield, CG

Hoffmann, B Benndorf, K Biskup, C

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Publication Type:: Conference Proceeding
Citation:: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2010, 7569
Issue Date:: 2010-05-07

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Field	Value	Language
dc.contributor.author	Dietrich, S	en_US
dc.contributor.author	Stanca, SE	en_US
dc.contributor.author	Cranfield, CG https://orcid.org/0000-0003-3608-5440	en_US
dc.contributor.author	Hoffmann, B	en_US
dc.contributor.author	Benndorf, K	en_US
dc.contributor.author	Biskup, C	en_US
dc.date.issued	2010-05-07	en_US
dc.identifier.citation	Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2010, 7569	en_US
dc.identifier.isbn	9780819479655	en_US
dc.identifier.issn	1605-7422	en_US
dc.identifier.uri	http://hdl.handle.net/10453/32795
dc.description.abstract	Fluorescent ion indicators are widely used to measure ion concentrations in living cells. However, despite considerable efforts in synthesizing new compounds, no ratiometric sodium indicator is available that can be excited at visible wavelengths. Ratiometric indicators have an advantage in that measured fluorescence intensities can be corrected for fluctuations of the indicator concentration and the illumination intensity, which is not possible when non-ratiometric indicators are used. One way to circumvent this problem is to measure fluorescence lifetimes, which are independent of these factors. Another way to overcome the disadvantages of a non-ratiometric indicator dye is to embed it, together with a reference dye, into nanoparticles. By relating the indicator fluorescence to the fluorescence of the reference dye, inhomogeneities in the nanosensor concentration or the illumination intensity can be cancelled out reliably. In this study we compare the benefits and drawbacks of these approaches. © 2010 Copyright SPIE - The International Society for Optical Engineering.	en_US
dc.relation.ispartof	Progress in Biomedical Optics and Imaging - Proceedings of SPIE	en_US
dc.relation.isbasedon	10.1117/12.849742	en_US
dc.title	New strategies to measure intracellular sodium concentrations	en_US
dc.type	Conference Proceeding
utslib.citation.volume	7569	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	7569	en_US

Abstract:

Fluorescent ion indicators are widely used to measure ion concentrations in living cells. However, despite considerable efforts in synthesizing new compounds, no ratiometric sodium indicator is available that can be excited at visible wavelengths. Ratiometric indicators have an advantage in that measured fluorescence intensities can be corrected for fluctuations of the indicator concentration and the illumination intensity, which is not possible when non-ratiometric indicators are used. One way to circumvent this problem is to measure fluorescence lifetimes, which are independent of these factors. Another way to overcome the disadvantages of a non-ratiometric indicator dye is to embed it, together with a reference dye, into nanoparticles. By relating the indicator fluorescence to the fluorescence of the reference dye, inhomogeneities in the nanosensor concentration or the illumination intensity can be cancelled out reliably. In this study we compare the benefits and drawbacks of these approaches. © 2010 Copyright SPIE - The International Society for Optical Engineering.

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http://hdl.handle.net/10453/32795