Formal revision of the alexandrium tamarense species complex (dinophyceae) taxonomy: The introduction of five species with emphasis on molecular-based (rDNA) classification

John, U; Litaker, RW; Montresor, M; Murray, S; Brosnahan, ML; Anderson, DM

Formal revision of the alexandrium tamarense species complex (dinophyceae) taxonomy: The introduction of five species with emphasis on molecular-based (rDNA) classification

John, U Litaker, RW Montresor, M Murray, S

Brosnahan, ML Anderson, DM

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Publication Type:: Journal Article
Citation:: Protist, 2014, 165 (6), pp. 779 - 804
Issue Date:: 2014-12-01

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Field	Value	Language
dc.contributor.author	John, U	en_US
dc.contributor.author	Litaker, RW	en_US
dc.contributor.author	Montresor, M	en_US
dc.contributor.author	Murray, S https://orcid.org/0000-0001-7096-1307	en_US
dc.contributor.author	Brosnahan, ML	en_US
dc.contributor.author	Anderson, DM	en_US
dc.date.available	2014-10-02	en_US
dc.date.issued	2014-12-01	en_US
dc.identifier.citation	Protist, 2014, 165 (6), pp. 779 - 804	en_US
dc.identifier.issn	1434-4610	en_US
dc.identifier.uri	http://hdl.handle.net/10453/33576
dc.description.abstract	© 2014 Elsevier GmbH. The Alexandrium tamarense species complex is one of the most studied marine dinoflagellate groups due to its ecological, toxicological and economic importance. Several members of this complex produce saxitoxin and its congeners - potent neurotoxins that cause paralytic shellfish poisoning. Isolates from this complex are assigned to A. tamarense, A. fundyense, or A. catenella based on two main morphological characters: the ability to form chains and the presence/absence of a ventral pore between Plates 1' and 4'. However, studies have shown that these characters are not consistent and/or distinctive. Further, phylogenies based on multiple regions in the rDNA operon indicate that the sequences from morphologically indistinguishable isolates partition into five clades. These clades were initially named based on their presumed geographic distribution, but recently were renamed as Groups I-V following the discovery of sympatry among some groups. In this study we present data on morphology, ITS/5.8S genetic distances, ITS2 compensatory base changes, mating incompatibilities, toxicity, the sxtA toxin synthesis gene, and rDNA phylogenies. All results were consistent with each group representing a distinct cryptic species. Accordingly, the groups were assigned species names as follows: Group I, A. fundyense; Group II, A. mediterraneum; Group III, A. tamarense; Group IV, A. pacificum; Group V, A. australiense.	en_US
dc.relation.ispartof	Protist	en_US
dc.relation.isbasedon	10.1016/j.protis.2014.10.001	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Dinoflagellida	en_US
dc.subject.mesh	Saxitoxin	en_US
dc.subject.mesh	DNA, Ribosomal	en_US
dc.subject.mesh	Genetic Markers	en_US
dc.subject.mesh	Marine Toxins	en_US
dc.subject.mesh	Neurotoxins	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Species Specificity	en_US
dc.subject.mesh	Databases, Genetic	en_US
dc.title	Formal revision of the alexandrium tamarense species complex (dinophyceae) taxonomy: The introduction of five species with emphasis on molecular-based (rDNA) classification	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	165	en_US
utslib.for	060405 Gene Expression (incl. Microarray and other genome-wide approaches)	en_US
utslib.for	060409 Molecular Evolution	en_US
utslib.for	060701 Phycology (incl. Marine Grasses)	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	165	en_US

Abstract:

© 2014 Elsevier GmbH. The Alexandrium tamarense species complex is one of the most studied marine dinoflagellate groups due to its ecological, toxicological and economic importance. Several members of this complex produce saxitoxin and its congeners - potent neurotoxins that cause paralytic shellfish poisoning. Isolates from this complex are assigned to A. tamarense, A. fundyense, or A. catenella based on two main morphological characters: the ability to form chains and the presence/absence of a ventral pore between Plates 1' and 4'. However, studies have shown that these characters are not consistent and/or distinctive. Further, phylogenies based on multiple regions in the rDNA operon indicate that the sequences from morphologically indistinguishable isolates partition into five clades. These clades were initially named based on their presumed geographic distribution, but recently were renamed as Groups I-V following the discovery of sympatry among some groups. In this study we present data on morphology, ITS/5.8S genetic distances, ITS2 compensatory base changes, mating incompatibilities, toxicity, the sxtA toxin synthesis gene, and rDNA phylogenies. All results were consistent with each group representing a distinct cryptic species. Accordingly, the groups were assigned species names as follows: Group I, A. fundyense; Group II, A. mediterraneum; Group III, A. tamarense; Group IV, A. pacificum; Group V, A. australiense.

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http://hdl.handle.net/10453/33576