Detachment and flow cytometric quantification of seagrass-associated bacteria

Publisher:
Elsevier
Publication Type:
Journal Article
Citation:
Journal of Microbiological Methods, 2014, 102 pp. 23 - 25
Issue Date:
2014-01
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A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0 × 105 and 8.0 × 106 cells mg- 1 DW tissue.
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