Detachment and flow cytometric quantification of seagrass-associated bacteria

Trevathan-Tackett, S; Macreadie, P; Ralph, P; Seymour, J

Detachment and flow cytometric quantification of seagrass-associated bacteria

Trevathan-Tackett, S

Macreadie, P

Ralph, P

Seymour, J

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Publication Type:: Journal Article
Citation:: Journal of Microbiological Methods, 2014, 102 pp. 23 - 25
Issue Date:: 2014-01-01

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Field	Value	Language
dc.contributor.author	Trevathan-Tackett, S https://orcid.org/0000-0002-4977-0757	en_US
dc.contributor.author	Macreadie, P https://orcid.org/0000-0001-7362-0882	en_US
dc.contributor.author	Ralph, P https://orcid.org/0000-0002-3103-7346	en_US
dc.contributor.author	Seymour, J https://orcid.org/0000-0002-3745-6541	en_US
dc.date.available	2014-04-14	en_US
dc.date.issued	2014-01-01	en_US
dc.identifier.citation	Journal of Microbiological Methods, 2014, 102 pp. 23 - 25	en_US
dc.identifier.issn	0167-7012	en_US
dc.identifier.uri	http://hdl.handle.net/10453/34084
dc.description.abstract	A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0×105 and 8.0×106cellsmg-1 DW tissue. © 2014 Elsevier B.V.	en_US
dc.relation.ispartof	Journal of Microbiological Methods	en_US
dc.relation.isbasedon	10.1016/j.mimet.2014.04.008	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Zosteraceae	en_US
dc.subject.mesh	Polysorbates	en_US
dc.subject.mesh	Detergents	en_US
dc.subject.mesh	Flow Cytometry	en_US
dc.subject.mesh	Stress, Mechanical	en_US
dc.subject.mesh	Bacterial Load	en_US
dc.title	Detachment and flow cytometric quantification of seagrass-associated bacteria	en_US
dc.type	Journal Article
utslib.citation.volume	102	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	102	en_US

Abstract:

A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0×105 and 8.0×106cellsmg-1 DW tissue. © 2014 Elsevier B.V.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/34084