Evaluation of the EasyScreen™ Enteric Parasite Detection Kit for the detection of Blastocystis spp., Cryptosporidium spp., Dientamoeba fragilis, Entamoeba complex, and Giardia intestinalis from clinical stool samples

Stark, D; Roberts, T; Ellis, JT; Marriott, D; Harkness, J

Evaluation of the EasyScreen™ Enteric Parasite Detection Kit for the detection of Blastocystis spp., Cryptosporidium spp., Dientamoeba fragilis, Entamoeba complex, and Giardia intestinalis from clinical stool samples

Stark, D Roberts, T

Ellis, JT

Marriott, D Harkness, J

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Publication Type:: Journal Article
Citation:: Diagnostic Microbiology and Infectious Disease, 2014, 78 (2), pp. 149 - 152
Issue Date:: 2014-02-01

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Field	Value	Language
dc.contributor.author	Stark, D	en_US
dc.contributor.author	Roberts, T https://orcid.org/0000-0001-8599-737X	en_US
dc.contributor.author	Ellis, JT https://orcid.org/0000-0001-7328-4831	en_US
dc.contributor.author	Marriott, D	en_US
dc.contributor.author	Harkness, J	en_US
dc.date.available	2013-10-15	en_US
dc.date.issued	2014-02-01	en_US
dc.identifier.citation	Diagnostic Microbiology and Infectious Disease, 2014, 78 (2), pp. 149 - 152	en_US
dc.identifier.issn	0732-8893	en_US
dc.identifier.uri	http://hdl.handle.net/10453/34141
dc.identifier.uri	http://hdl.handle.net/10453/28481
dc.description.abstract	The aim of this study was to evaluate the EasyScreen™ Enteric Parasite Detection Kit (Genetic Signatures, Sydney, Australia) for the detection and identification of 5 common enteric parasites: Blastocystis spp., Cryptosporidium spp., Dientamoeba fragilis, Entamoeba complex, and Giardia intestinalis in human clinical samples. A total of 358 faecal samples were included in the study. When compared to real-time PCR and microscopy, the EasyScreen™ Enteric Parasite Detection Kit exhibited 92-100% sensitivity and 100% specificity and detected all commonly found genotypes and subtypes of clinically important human parasites. No cross reactivity was detected in stool samples containing various other bacterial, viral, and/or protozoan species. The EasyScreen™ PCR assay was able to provide rapid, sensitive, and specific simultaneous detection and identification of the 5 most important diarrhoea-causing enteric parasites that infect humans. It should be noted, however, that the EasyScreen™ Kit does not substitute for microscopy or for additional PCRs as it does not detect the pathogenic Coccidia spp. Cystoisospora belli or Cyclospora cayetanensis and it does not differentiate between pathogenic and nonpathogenic Entamoeba spp. This study also highlights the lack of sensitivity demonstrated by microscopy; as such, molecular methods should be considered the diagnostic method of choice for enteric parasites. © 2014 Elsevier Inc.	en_US
dc.relation.ispartof	Diagnostic Microbiology and Infectious Disease	en_US
dc.relation.isbasedon	10.1016/j.diagmicrobio.2013.10.013	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Feces	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Parasites	en_US
dc.subject.mesh	Intestinal Diseases, Parasitic	en_US
dc.subject.mesh	Reagent Kits, Diagnostic	en_US
dc.subject.mesh	Microscopy	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.title	Evaluation of the EasyScreen™ Enteric Parasite Detection Kit for the detection of Blastocystis spp., Cryptosporidium spp., Dientamoeba fragilis, Entamoeba complex, and Giardia intestinalis from clinical stool samples	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	78	en_US
utslib.for	110803 Medical Parasitology	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1103 Clinical Sciences	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	78	en_US

Abstract:

The aim of this study was to evaluate the EasyScreen™ Enteric Parasite Detection Kit (Genetic Signatures, Sydney, Australia) for the detection and identification of 5 common enteric parasites: Blastocystis spp., Cryptosporidium spp., Dientamoeba fragilis, Entamoeba complex, and Giardia intestinalis in human clinical samples. A total of 358 faecal samples were included in the study. When compared to real-time PCR and microscopy, the EasyScreen™ Enteric Parasite Detection Kit exhibited 92-100% sensitivity and 100% specificity and detected all commonly found genotypes and subtypes of clinically important human parasites. No cross reactivity was detected in stool samples containing various other bacterial, viral, and/or protozoan species. The EasyScreen™ PCR assay was able to provide rapid, sensitive, and specific simultaneous detection and identification of the 5 most important diarrhoea-causing enteric parasites that infect humans. It should be noted, however, that the EasyScreen™ Kit does not substitute for microscopy or for additional PCRs as it does not detect the pathogenic Coccidia spp. Cystoisospora belli or Cyclospora cayetanensis and it does not differentiate between pathogenic and nonpathogenic Entamoeba spp. This study also highlights the lack of sensitivity demonstrated by microscopy; as such, molecular methods should be considered the diagnostic method of choice for enteric parasites. © 2014 Elsevier Inc.

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http://hdl.handle.net/10453/28481