Cytokine mRNA expression responses to resistance, aerobic, and concurrent exercise in sedentary middle-aged men

Donges, CE; Duffield, R; Smith, GC; Short, MJ; Edge, JA

Cytokine mRNA expression responses to resistance, aerobic, and concurrent exercise in sedentary middle-aged men

Donges, CE Duffield, R

Smith, GC Short, MJ Edge, JA

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Publication Type:: Journal Article
Citation:: Applied Physiology, Nutrition and Metabolism, 2014, 39 (2), pp. 130 - 137
Issue Date:: 2014-02-05

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Field	Value	Language
dc.contributor.author	Donges, CE	en_US
dc.contributor.author	Duffield, R https://orcid.org/0000-0002-5641-1314	en_US
dc.contributor.author	Smith, GC	en_US
dc.contributor.author	Short, MJ	en_US
dc.contributor.author	Edge, JA	en_US
dc.date.issued	2014-02-05	en_US
dc.identifier.citation	Applied Physiology, Nutrition and Metabolism, 2014, 39 (2), pp. 130 - 137	en_US
dc.identifier.issn	1715-5312	en_US
dc.identifier.uri	http://hdl.handle.net/10453/34279
dc.description.abstract	Concurrent resistance and aerobic exercise (CE) is recommended to ageing populations, though is postulated to induce diminished acute molecular responses. Given that contraction-induced cytokine mRNA expression reportedly mediates remunerative postexercise molecular responses, it is necessary to determine whether cytokine mRNA expression may be diminished after CE. Eight middle-aged men (age, 53.3 ±1.8 years; body mass index, 29.4 ± 1.4 kg·m-2) randomly completed (balanced for completion order) 8 × 8 leg extensions at 70% maximal strength (RE), 40 min of cycling at 55% of peak aerobic workload (AE), or (workload-matched) 50% RE and 50% AE (CE). Muscle (vastus lateralis) was obtained pre-exercise, and at 1 h and 4 h postexercise, and analyzed for changes of glycogen concentration, tumor necrosis factor (TNF)α, TNF receptor-1 and -2 (TNF-R1 and TNF-R2, respectively), interleukin (IL)-6, IL-6R, IL-1β, and IL-1 receptor-antagonist (IL-1ra). All exercise modes upregulated cytokine mRNA expression at 1hpostexercise comparably (TNFα, TNF-R1, TNF-R2, IL-1β, IL-6) (p<0.05). Expression remained elevated at 4hafter RE and AE (p < 0.05), though returned to pre-exercise levels after CE (p > 0.05). Moreover, AE and RE upregulated IL-1β and IL-1ra expression, whereas CE upregulated IL-1β expression only (p < 0.05). Only AE reduced muscle glycogen concentration (p < 0.05), whilst upregulating receptor expression the greatest; though, IL-6R expression remained unchanged after all modes (p > 0.05). In conclusion, in middle-aged men, all modes induced commensurate cytokine mRNA expression at 1 h postexercise; however, only CE resulted in ameliorated expression at 4 h postexercise. Whether the RE or AE components of CE are independently or cumulatively sufficient to upregulate cytokine responses, or whether they collectively inhibit cytokine mRNA expression, remains to be determined.	en_US
dc.relation.ispartof	Applied Physiology, Nutrition and Metabolism	en_US
dc.relation.isbasedon	10.1139/apnm-2013-0076	en_US
dc.subject.classification	Sport Sciences	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	RNA, Messenger	en_US
dc.subject.mesh	Cytokines	en_US
dc.subject.mesh	Exercise	en_US
dc.subject.mesh	Age Factors	en_US
dc.subject.mesh	Middle Aged	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Resistance Training	en_US
dc.subject.mesh	Sedentary Lifestyle	en_US
dc.subject.mesh	Sedentary Behavior	en_US
dc.title	Cytokine mRNA expression responses to resistance, aerobic, and concurrent exercise in sedentary middle-aged men	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	39	en_US
utslib.for	110602 Exercise Physiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Sports and Exercise Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHSP - Health Services and Practice
utslib.copyright.status	open_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	39	en_US

Abstract:

Concurrent resistance and aerobic exercise (CE) is recommended to ageing populations, though is postulated to induce diminished acute molecular responses. Given that contraction-induced cytokine mRNA expression reportedly mediates remunerative postexercise molecular responses, it is necessary to determine whether cytokine mRNA expression may be diminished after CE. Eight middle-aged men (age, 53.3 ±1.8 years; body mass index, 29.4 ± 1.4 kg·m-2) randomly completed (balanced for completion order) 8 × 8 leg extensions at 70% maximal strength (RE), 40 min of cycling at 55% of peak aerobic workload (AE), or (workload-matched) 50% RE and 50% AE (CE). Muscle (vastus lateralis) was obtained pre-exercise, and at 1 h and 4 h postexercise, and analyzed for changes of glycogen concentration, tumor necrosis factor (TNF)α, TNF receptor-1 and -2 (TNF-R1 and TNF-R2, respectively), interleukin (IL)-6, IL-6R, IL-1β, and IL-1 receptor-antagonist (IL-1ra). All exercise modes upregulated cytokine mRNA expression at 1hpostexercise comparably (TNFα, TNF-R1, TNF-R2, IL-1β, IL-6) (p<0.05). Expression remained elevated at 4hafter RE and AE (p < 0.05), though returned to pre-exercise levels after CE (p > 0.05). Moreover, AE and RE upregulated IL-1β and IL-1ra expression, whereas CE upregulated IL-1β expression only (p < 0.05). Only AE reduced muscle glycogen concentration (p < 0.05), whilst upregulating receptor expression the greatest; though, IL-6R expression remained unchanged after all modes (p > 0.05). In conclusion, in middle-aged men, all modes induced commensurate cytokine mRNA expression at 1 h postexercise; however, only CE resulted in ameliorated expression at 4 h postexercise. Whether the RE or AE components of CE are independently or cumulatively sufficient to upregulate cytokine responses, or whether they collectively inhibit cytokine mRNA expression, remains to be determined.

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http://hdl.handle.net/10453/34279