A TaqMan qPCR method for detecting kdr resistance in Aphis gossypii demonstrates improved sensitivity compared to conventional PCR–RFLP

Suann, M; Bogema, DR; Chen, Y; Mansfield, S; Barchia, IM; Herron, GA

A TaqMan qPCR method for detecting kdr resistance in Aphis gossypii demonstrates improved sensitivity compared to conventional PCR–RFLP

Suann, M Bogema, DR

Chen, Y Mansfield, S Barchia, IM Herron, GA

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Publication Type:: Journal Article
Citation:: Journal of Pest Science, 2015, 88 (4), pp. 785 - 791
Issue Date:: 2015-02-25

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Field	Value	Language
dc.contributor.author	Suann, M	en_US
dc.contributor.author	Bogema, DR https://orcid.org/0000-0001-7013-7326	en_US
dc.contributor.author	Chen, Y	en_US
dc.contributor.author	Mansfield, S	en_US
dc.contributor.author	Barchia, IM	en_US
dc.contributor.author	Herron, GA	en_US
dc.date.issued	2015-02-25	en_US
dc.identifier.citation	Journal of Pest Science, 2015, 88 (4), pp. 785 - 791	en_US
dc.identifier.issn	1612-4758	en_US
dc.identifier.uri	http://hdl.handle.net/10453/34341
dc.description.abstract	© 2015, Springer-Verlag Berlin Heidelberg. Cotton aphid, Aphis gossypii Glover, has emerged as a prominent pest in Australian cotton production, and monitoring pesticide resistance including pyrethroids in field populations is crucial for its sustainable management. We examined the distribution of kdr resistance in 35 field-collected A. gossypii populations and used TaqMan qPCR assays with pooled samples. The study demonstrated proof of concept that pooled insect qPCR methodology provided effective detection with better sensitivity than individual PCR–RFLP genotyping techniques for the kdr resistance allele. The practical outcome is that routine resistance monitoring can examine more sites while increasing the likelihood of detecting incipient resistance at those sites. More importantly, the method is adaptable to any genetically caused resistance and so not limited to A. gossypii or even insect control. It cannot be overstressed that the ability to detected resistance at very low frequencies is critical to all sustainable resistance management. Early detection of resistance provides critical time for the modification of chemical use prior to potential insecticide control failure.	en_US
dc.relation.ispartof	Journal of Pest Science	en_US
dc.relation.isbasedon	10.1007/s10340-015-0651-5	en_US
dc.subject.classification	Forestry	en_US
dc.subject.classification	Entomology	en_US
dc.title	A TaqMan qPCR method for detecting kdr resistance in Aphis gossypii demonstrates improved sensitivity compared to conventional PCR–RFLP	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	88	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	88	en_US

Abstract:

© 2015, Springer-Verlag Berlin Heidelberg. Cotton aphid, Aphis gossypii Glover, has emerged as a prominent pest in Australian cotton production, and monitoring pesticide resistance including pyrethroids in field populations is crucial for its sustainable management. We examined the distribution of kdr resistance in 35 field-collected A. gossypii populations and used TaqMan qPCR assays with pooled samples. The study demonstrated proof of concept that pooled insect qPCR methodology provided effective detection with better sensitivity than individual PCR–RFLP genotyping techniques for the kdr resistance allele. The practical outcome is that routine resistance monitoring can examine more sites while increasing the likelihood of detecting incipient resistance at those sites. More importantly, the method is adaptable to any genetically caused resistance and so not limited to A. gossypii or even insect control. It cannot be overstressed that the ability to detected resistance at very low frequencies is critical to all sustainable resistance management. Early detection of resistance provides critical time for the modification of chemical use prior to potential insecticide control failure.

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http://hdl.handle.net/10453/34341