The effect of diel temperature and light cycles on the growth of Nannochloropsis oculata in a photobioreactor matrix

Tamburic, B; Guruprasad, S; Radford, DT; Szabó, M; Lilley, RMC; Larkum, AWD; Franklin, JB; Kramer, DM; Blackburn, SI; Raven, JA; Schliep, M; Ralph, PJ

The effect of diel temperature and light cycles on the growth of Nannochloropsis oculata in a photobioreactor matrix

Tamburic, B

Guruprasad, S Radford, DT Szabó, M

Lilley, RMC Larkum, AWD

Franklin, JB Kramer, DM

Blackburn, SI Raven, JA

Schliep, M Ralph, PJ

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Publication Type:: Journal Article
Citation:: PLoS ONE, 2014, 9 (1)
Issue Date:: 2014-01-20

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Field	Value	Language
dc.contributor.author	Tamburic, B https://orcid.org/0000-0001-5720-9380	en_US
dc.contributor.author	Guruprasad, S	en_US
dc.contributor.author	Radford, DT	en_US
dc.contributor.author	Szabó, M https://orcid.org/0000-0001-6235-2894	en_US
dc.contributor.author	Lilley, RMC	en_US
dc.contributor.author	Larkum, AWD https://orcid.org/0000-0002-0420-134X	en_US
dc.contributor.author	Franklin, JB	en_US
dc.contributor.author	Kramer, DM https://orcid.org/0000-0003-2181-6888	en_US
dc.contributor.author	Blackburn, SI	en_US
dc.contributor.author	Raven, JA https://orcid.org/0000-0002-2789-3297	en_US
dc.contributor.author	Schliep, M	en_US
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.date.available	2013-12-04	en_US
dc.date.issued	2014-01-20	en_US
dc.identifier.citation	PLoS ONE, 2014, 9 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/34699
dc.description.abstract	A matrix of photobioreactors integrated with metabolic sensors was used to examine the combined impact of light and temperature variations on the growth and physiology of the biofuel candidate microalgal species Nannochloropsis oculata. The experiments were performed with algal cultures maintained at a constant 20u C versus a 15°C to 25°C diel temperature cycle, where light intensity also followed a diel cycle with a maximum irradiance of 1920 μmol photons m-2 s<sup>-1</sup>. No differences in algal growth (Chlorophyll a) were found between the two environmental regimes; however, the metabolic processes responded differently throughout the day to the change in environmental conditions. The variable temperature treatment resulted in greater damage to photosystem II due to the combined effect of strong light and high temperature. Cellular functions responded differently to conditions before midday as opposed to the afternoon, leading to strong hysteresis in dissolved oxygen concentration, quantum yield of photosystem II and net photosynthesis. Overnight metabolism performed differently, probably as a result of the temperature impact on respiration. Our photobioreactor matrix has produced novel insights into the physiological response of Nannochloropsis oculata to simulated environmental conditions. This information can be used to predict the effectiveness of deploying Nannochloropsis oculata in similar field conditions for commercial biofuel production. © 2014 Tamburic et al.	en_US
dc.relation.ispartof	PLoS ONE	en_US
dc.relation.isbasedon	10.1371/journal.pone.0086047	en_US
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Chlorophyll	en_US
dc.subject.mesh	Equipment Design	en_US
dc.subject.mesh	Temperature	en_US
dc.subject.mesh	Photosynthesis	en_US
dc.subject.mesh	Light	en_US
dc.subject.mesh	Photoperiod	en_US
dc.subject.mesh	Biofuels	en_US
dc.subject.mesh	Microalgae	en_US
dc.subject.mesh	Photobioreactors	en_US
dc.subject.mesh	Chlorophyll A	en_US
dc.title	The effect of diel temperature and light cycles on the growth of Nannochloropsis oculata in a photobioreactor matrix	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	9	en_US
utslib.for	0607 Plant Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

A matrix of photobioreactors integrated with metabolic sensors was used to examine the combined impact of light and temperature variations on the growth and physiology of the biofuel candidate microalgal species Nannochloropsis oculata. The experiments were performed with algal cultures maintained at a constant 20u C versus a 15°C to 25°C diel temperature cycle, where light intensity also followed a diel cycle with a maximum irradiance of 1920 μmol photons m-2 s^-1. No differences in algal growth (Chlorophyll a) were found between the two environmental regimes; however, the metabolic processes responded differently throughout the day to the change in environmental conditions. The variable temperature treatment resulted in greater damage to photosystem II due to the combined effect of strong light and high temperature. Cellular functions responded differently to conditions before midday as opposed to the afternoon, leading to strong hysteresis in dissolved oxygen concentration, quantum yield of photosystem II and net photosynthesis. Overnight metabolism performed differently, probably as a result of the temperature impact on respiration. Our photobioreactor matrix has produced novel insights into the physiological response of Nannochloropsis oculata to simulated environmental conditions. This information can be used to predict the effectiveness of deploying Nannochloropsis oculata in similar field conditions for commercial biofuel production. © 2014 Tamburic et al.

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http://hdl.handle.net/10453/34699