Photosynthetic acclimation of Nannochloropsis oculata investigated by multi-wavelength chlorophyll fluorescence analysis

Szabó, M; Parker, K; Guruprasad, S; Kuzhiumparambil, U; Lilley, RMC; Tamburic, B; Schliep, M; Larkum, AWD; Schreiber, U; Raven, JA; Ralph, PJ

Photosynthetic acclimation of Nannochloropsis oculata investigated by multi-wavelength chlorophyll fluorescence analysis

Szabó, M

Parker, K Guruprasad, S Kuzhiumparambil, U

Lilley, RMC Tamburic, B

Schliep, M Larkum, AWD

Schreiber, U Raven, JA

Ralph, PJ

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Publication Type:: Journal Article
Citation:: Bioresource Technology, 2014, 167 pp. 521 - 529
Issue Date:: 2014-01-01

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Field	Value	Language
dc.contributor.author	Szabó, M https://orcid.org/0000-0001-6235-2894	en_US
dc.contributor.author	Parker, K	en_US
dc.contributor.author	Guruprasad, S	en_US
dc.contributor.author	Kuzhiumparambil, U https://orcid.org/0000-0003-0582-6779	en_US
dc.contributor.author	Lilley, RMC	en_US
dc.contributor.author	Tamburic, B https://orcid.org/0000-0001-5720-9380	en_US
dc.contributor.author	Schliep, M	en_US
dc.contributor.author	Larkum, AWD https://orcid.org/0000-0002-0420-134X	en_US
dc.contributor.author	Schreiber, U	en_US
dc.contributor.author	Raven, JA https://orcid.org/0000-0002-2789-3297	en_US
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.date.available	2020-05-25T19:07:52Z
dc.date.issued	2014-01-01	en_US
dc.identifier.citation	Bioresource Technology, 2014, 167 pp. 521 - 529	en_US
dc.identifier.issn	0960-8524	en_US
dc.identifier.uri	http://hdl.handle.net/10453/34701
dc.description.abstract	Multi-wavelength chlorophyll fluorescence analysis was utilised to examine the photosynthetic efficiency of the biofuel-producing alga Nannochloropsis oculata, grown under two light regimes; low (LL) and high (HL) irradiance levels. Wavelength dependency was evident in the functional absorption cross-section of Photosystem II (σII(λ)), absolute electron transfer rates (ETR(II)), and non-photochemical quenching (NPQ) of chlorophyll fluorescence in both HL and LL cells. While σII(λ) was not significantly different between the two growth conditions, HL cells upregulated ETR(II) 1.6-1.8-fold compared to LL cells, most significantly in the wavelength range of 440-540nm. This indicates preferential utilisation of blue-green light, a highly relevant spectral region for visible light in algal pond conditions. Under these conditions, the HL cells accumulated saturated fatty acids, whereas polyunsaturated fatty acids were more abundant in LL cells. This knowledge is of importance for the use of N. oculata for fatty acid production in the biofuel industry. © 2014 Elsevier Ltd.	en_US
dc.relation.ispartof	Bioresource Technology	en_US
dc.relation.isbasedon	10.1016/j.biortech.2014.06.046	en_US
dc.subject.classification	Biotechnology	en_US
dc.subject.mesh	Esters	en_US
dc.subject.mesh	Chlorophyll	en_US
dc.subject.mesh	Photosystem II Protein Complex	en_US
dc.subject.mesh	Fatty Acids	en_US
dc.subject.mesh	Acclimatization	en_US
dc.subject.mesh	Photosynthesis	en_US
dc.subject.mesh	Electron Transport	en_US
dc.subject.mesh	Quantum Theory	en_US
dc.subject.mesh	Fluorescence	en_US
dc.subject.mesh	Microalgae	en_US
dc.subject.mesh	Absorption, Physicochemical	en_US
dc.title	Photosynthetic acclimation of Nannochloropsis oculata investigated by multi-wavelength chlorophyll fluorescence analysis	en_US
dc.type	Journal Article
utslib.citation.volume	167	en_US
utslib.for	050102 Ecosystem Function	en_US
utslib.for	060104 Cell Metabolism	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	167	en_US

Abstract:

Multi-wavelength chlorophyll fluorescence analysis was utilised to examine the photosynthetic efficiency of the biofuel-producing alga Nannochloropsis oculata, grown under two light regimes; low (LL) and high (HL) irradiance levels. Wavelength dependency was evident in the functional absorption cross-section of Photosystem II (σII(λ)), absolute electron transfer rates (ETR(II)), and non-photochemical quenching (NPQ) of chlorophyll fluorescence in both HL and LL cells. While σII(λ) was not significantly different between the two growth conditions, HL cells upregulated ETR(II) 1.6-1.8-fold compared to LL cells, most significantly in the wavelength range of 440-540nm. This indicates preferential utilisation of blue-green light, a highly relevant spectral region for visible light in algal pond conditions. Under these conditions, the HL cells accumulated saturated fatty acids, whereas polyunsaturated fatty acids were more abundant in LL cells. This knowledge is of importance for the use of N. oculata for fatty acid production in the biofuel industry. © 2014 Elsevier Ltd.

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http://hdl.handle.net/10453/34701