Determination of selenium in serum in the presence of gadolinium with ICP-QQQ-MS

Bishop, DP; Hare, DJ; Fryer, F; Taudte, RV; Cardoso, BR; Cole, N; Doble, PA

Determination of selenium in serum in the presence of gadolinium with ICP-QQQ-MS

Bishop, DP

Hare, DJ

Fryer, F Taudte, RV

Cardoso, BR Cole, N

Doble, PA

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Publication Type:: Journal Article
Citation:: Analyst, 2015, 140 (8), pp. 2842 - 2846
Issue Date:: 2015-04-21

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Field	Value	Language
dc.contributor.author	Bishop, DP https://orcid.org/0000-0002-6533-4410	en_US
dc.contributor.author	Hare, DJ https://orcid.org/0000-0002-5922-7643	en_US
dc.contributor.author	Fryer, F	en_US
dc.contributor.author	Taudte, RV https://orcid.org/0000-0003-4617-6063	en_US
dc.contributor.author	Cardoso, BR	en_US
dc.contributor.author	Cole, N https://orcid.org/0000-0002-9866-4694	en_US
dc.contributor.author	Doble, PA https://orcid.org/0000-0002-8472-1301	en_US
dc.date.issued	2015-04-21	en_US
dc.identifier.citation	Analyst, 2015, 140 (8), pp. 2842 - 2846	en_US
dc.identifier.issn	0003-2654	en_US
dc.identifier.uri	http://hdl.handle.net/10453/35235
dc.description.abstract	This journal is © The Royal Society of Chemistry 2015. Gadolinium (Gd)-based magnetic resonance imaging (MRI) contrasting agents interfere with the determination of selenium (Se) when analysed by single quadrupole inductively coupled plasma-mass spectrometry (ICP-MS). This paper demonstrates that an ICP-triple quadrupole-MS (ICP-QQQ-MS) with oxygen mass shift overcomes Gd++ interference on Se+ and mitigates typically encountered matrix and spectral based interferences. Normal human serum was diluted in a solution containing isopropanol, EDTA, NH4OH and Triton X-100. Samples were unspiked (control) serum; serum spiked with 0.127 μmol L-1 Se or 127 μmol L-1 Gd; and serum spiked with both 0.127 μmol L-1 Se and 127 μmol L-1 Gd. Consideration of collision/reaction gases and conditions for interference mitigation included helium (He); a 'low' and 'high' hydrogen (H2) flow, and oxygen (O2). The instrument tune for O2 was optimised for effective elimination of interferences via a mass shift reaction of Se+ to SeO+. The ICP-QQQ-MS was capable of detecting trace (>9.34 nmol L-1) levels of Se in serum in the presence of Gd in our simulated post-MRI serum sample. The multi-tune capabilities of the ICP-QQQ-MS may be adapted to eliminate other specific isobaric interferences that cause false positive results in other analyses where the analyte is confounded by doubly charged and/or polyatomic species.	en_US
dc.relation.ispartof	Analyst	en_US
dc.relation.isbasedon	10.1039/c4an02283a	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	Selenium	en_US
dc.subject.mesh	Gadolinium	en_US
dc.subject.mesh	Blood Chemical Analysis	en_US
dc.subject.mesh	Mass Spectrometry	en_US
dc.title	Determination of selenium in serum in the presence of gadolinium with ICP-QQQ-MS	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	140	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	140	en_US

Abstract:

This journal is © The Royal Society of Chemistry 2015. Gadolinium (Gd)-based magnetic resonance imaging (MRI) contrasting agents interfere with the determination of selenium (Se) when analysed by single quadrupole inductively coupled plasma-mass spectrometry (ICP-MS). This paper demonstrates that an ICP-triple quadrupole-MS (ICP-QQQ-MS) with oxygen mass shift overcomes Gd++ interference on Se+ and mitigates typically encountered matrix and spectral based interferences. Normal human serum was diluted in a solution containing isopropanol, EDTA, NH4OH and Triton X-100. Samples were unspiked (control) serum; serum spiked with 0.127 μmol L-1 Se or 127 μmol L-1 Gd; and serum spiked with both 0.127 μmol L-1 Se and 127 μmol L-1 Gd. Consideration of collision/reaction gases and conditions for interference mitigation included helium (He); a 'low' and 'high' hydrogen (H2) flow, and oxygen (O2). The instrument tune for O2 was optimised for effective elimination of interferences via a mass shift reaction of Se+ to SeO+. The ICP-QQQ-MS was capable of detecting trace (>9.34 nmol L-1) levels of Se in serum in the presence of Gd in our simulated post-MRI serum sample. The multi-tune capabilities of the ICP-QQQ-MS may be adapted to eliminate other specific isobaric interferences that cause false positive results in other analyses where the analyte is confounded by doubly charged and/or polyatomic species.

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http://hdl.handle.net/10453/35235