Determination of selenium in serum in the presence of gadolinium with ICP-QQQ-MS

Publication Type:
Journal Article
Citation:
Analyst, 2015, 140 (8), pp. 2842 - 2846
Issue Date:
2015-04-21
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This journal is © The Royal Society of Chemistry 2015. Gadolinium (Gd)-based magnetic resonance imaging (MRI) contrasting agents interfere with the determination of selenium (Se) when analysed by single quadrupole inductively coupled plasma-mass spectrometry (ICP-MS). This paper demonstrates that an ICP-triple quadrupole-MS (ICP-QQQ-MS) with oxygen mass shift overcomes Gd++interference on Se+and mitigates typically encountered matrix and spectral based interferences. Normal human serum was diluted in a solution containing isopropanol, EDTA, NH4OH and Triton X-100. Samples were unspiked (control) serum; serum spiked with 0.127 μmol L-1Se or 127 μmol L-1Gd; and serum spiked with both 0.127 μmol L-1Se and 127 μmol L-1Gd. Consideration of collision/reaction gases and conditions for interference mitigation included helium (He); a 'low' and 'high' hydrogen (H2) flow, and oxygen (O2). The instrument tune for O2was optimised for effective elimination of interferences via a mass shift reaction of Se+to SeO+. The ICP-QQQ-MS was capable of detecting trace (>9.34 nmol L-1) levels of Se in serum in the presence of Gd in our simulated post-MRI serum sample. The multi-tune capabilities of the ICP-QQQ-MS may be adapted to eliminate other specific isobaric interferences that cause false positive results in other analyses where the analyte is confounded by doubly charged and/or polyatomic species.
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