A split flow chamber with artificial sediment to examine the below-ground microenvironment of aquatic macrophytes

Publication Type:
Journal Article
Citation:
Marine Biology, 2014, 161 (12), pp. 2921 - 2930
Issue Date:
2014-01-01
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© 2014, Springer-Verlag Berlin Heidelberg. We present a new experimental set-up enabling fine-scale examination of how changing environmental conditions affect the below-ground biogeochemical microenvironment of aquatic macrophytes. By means of microsensor and planar optode technology, the influence of plant-mediated radial O 2 release on the below-ground chemical microenvironment of Zostera muelleri and Halophila ovalis was determined in high spatio-temporal resolution. The seagrass specimens were cultured in a new split flow chamber with artificial sediment made of a deoxygenated seawater–agar solution with added sulphide. Microelectrode measurements revealed radial O 2 release from the root–shoot junction of both Z. muelleri and H. ovalis during both light stimulation and darkness, resulting in a rapid decrease in H 2 S concentration, and a significant drop in pH was observed within the plant-derived oxic microzone of Z. muelleri. No radial O 2 release was detectable from the below-ground tissue of Z. muelleri during conditions of combined water-column hypoxia and darkness, leaving the plants more susceptible to sulphide invasion. The spatial O 2 heterogeneity within the immediate rhizosphere of Z. muelleri was furthermore determined in two dimensions by means of planar optodes. O 2 images revealed a decrease in the spatial extent of the plant-derived oxic microzone surrounding the below-ground tissue during darkness, supporting the microelectrode measurements. This new experimental approach can be applied to all rooted aquatic plants, as it allows for direct visual assessment of the below-ground tissue surface during microprofiling, while enabling modification of the above-ground environmental conditions.
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