Herbicide impact on Hormosira banksii gametes measured by fluorescence and germination bioassays

Seery, CR; Gunthorpe, L; Ralph, PJ

Herbicide impact on Hormosira banksii gametes measured by fluorescence and germination bioassays

Seery, CR Gunthorpe, L Ralph, PJ

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Publication Type:: Journal Article
Citation:: Environmental Pollution, 2006, 140 (1), pp. 43 - 51
Issue Date:: 2006-03-01

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Field	Value	Language
dc.contributor.author	Seery, CR	en_US
dc.contributor.author	Gunthorpe, L	en_US
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.date.available	2005-07-01	en_US
dc.date.issued	2006-03-01	en_US
dc.identifier.citation	Environmental Pollution, 2006, 140 (1), pp. 43 - 51	en_US
dc.identifier.issn	0269-7491	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3611
dc.description.abstract	The innovative bioassay described here involves chlorophyll a fluorescence measurements of gametes from the macroalgae, Hormosira banksii, where gametes (eggs) were exposed to Diuron, Irgarol and Bromacil. Response was assessed as percent inhibition from control of effective quantum yield (ΔF/Fm′) of photosystem II, herein referred to as % PSII Inhibition. This was measured with the dual-channelled pulse amplitude modulated (PAM) fluorometer, ToxY-PAM. The fluorescence bioassay was run simultaneously with an established H. banksii germination bioassay to compare sensitivity, precision, and time-to-result. The fluorescence bioassay gave highly sensitive results evidenced by EC 50s (% PSII Inhibition) for Diuron, Irgarol and Bromacil being three, four and three orders of magnitude (respectively) lower than EC50s generated from the germination bioassays. Precision of the fluorescence bioassay was demonstrated with low coefficient of variations (<30%) for all three toxicants. With regard to time, the fluorescence bioassay gave results within 6 h, as opposed to more than 50 h for the germination bioassay. © 2005 Elsevier Ltd. All rights reserved.	en_US
dc.relation	http://purl.org/au-research/grants/arc/C00107633
dc.relation.ispartof	Environmental Pollution	en_US
dc.relation.isbasedon	10.1016/j.envpol.2005.07.001	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.subject.mesh	Germ Cells	en_US
dc.subject.mesh	Diuron	en_US
dc.subject.mesh	Bromouracil	en_US
dc.subject.mesh	Chlorophyll	en_US
dc.subject.mesh	Triazines	en_US
dc.subject.mesh	Photosystem II Protein Complex	en_US
dc.subject.mesh	Herbicides	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Biological Assay	en_US
dc.subject.mesh	Toxicity Tests	en_US
dc.subject.mesh	Photosynthesis	en_US
dc.subject.mesh	Fluorescence	en_US
dc.subject.mesh	Phaeophyta	en_US
dc.subject.mesh	Chlorophyll A	en_US
dc.title	Herbicide impact on Hormosira banksii gametes measured by fluorescence and germination bioassays	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	140	en_US
utslib.for	0602 Ecology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	140	en_US

Abstract:

The innovative bioassay described here involves chlorophyll a fluorescence measurements of gametes from the macroalgae, Hormosira banksii, where gametes (eggs) were exposed to Diuron, Irgarol and Bromacil. Response was assessed as percent inhibition from control of effective quantum yield (ΔF/Fm′) of photosystem II, herein referred to as % PSII Inhibition. This was measured with the dual-channelled pulse amplitude modulated (PAM) fluorometer, ToxY-PAM. The fluorescence bioassay was run simultaneously with an established H. banksii germination bioassay to compare sensitivity, precision, and time-to-result. The fluorescence bioassay gave highly sensitive results evidenced by EC 50s (% PSII Inhibition) for Diuron, Irgarol and Bromacil being three, four and three orders of magnitude (respectively) lower than EC50s generated from the germination bioassays. Precision of the fluorescence bioassay was demonstrated with low coefficient of variations (<30%) for all three toxicants. With regard to time, the fluorescence bioassay gave results within 6 h, as opposed to more than 50 h for the germination bioassay. © 2005 Elsevier Ltd. All rights reserved.

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http://hdl.handle.net/10453/3611