Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator

Liu, Y; Ding, Y; Alonas, E; Zhao, W; Santangelo, PJ; Jin, D; Piper, JA; Teng, J; Ren, Q; Xi, P

Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator

Liu, Y Ding, Y Alonas, E Zhao, W Santangelo, PJ Jin, D

Piper, JA Teng, J Ren, Q Xi, P

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Publication Type:: Journal Article
Citation:: PLoS ONE, 2012, 7 (6)
Issue Date:: 2012-06-27

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Field	Value	Language
dc.contributor.author	Liu, Y	en_US
dc.contributor.author	Ding, Y	en_US
dc.contributor.author	Alonas, E	en_US
dc.contributor.author	Zhao, W	en_US
dc.contributor.author	Santangelo, PJ	en_US
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666	en_US
dc.contributor.author	Piper, JA	en_US
dc.contributor.author	Teng, J	en_US
dc.contributor.author	Ren, Q	en_US
dc.contributor.author	Xi, P	en_US
dc.date.available	2012-05-30	en_US
dc.date.issued	2012-06-27	en_US
dc.identifier.citation	PLoS ONE, 2012, 7 (6)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/37058
dc.description.abstract	In this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. With synchronization-free STED, we imaged 200 nm nanospheres as well as all three cytoskeletal elements (microtubules, intermediate filaments, and actin filaments), clearly demonstrating the resolving power of synchronization-free STED over conventional diffraction limited imaging. It also allowed us to discover that, Dylight 650, exhibits improved performance over ATTO647N, a fluorophore frequently used in STED. Furthermore, we applied synchronization-free STED to image fluorescently-labeled intracellular viral RNA granules, which otherwise cannot be differentiated by confocal microscopy. Thanks to the widely available Ti:Sapphire oscillators in multiphoton imaging system, this work suggests easier access to setup super-resolution microscope via the synchronization-free STED. © 2012 Liu et al.	en_US
dc.relation.ispartof	PLoS ONE	en_US
dc.relation.isbasedon	10.1371/journal.pone.0040003	en_US
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Cytoskeleton	en_US
dc.subject.mesh	Titanium	en_US
dc.subject.mesh	Microscopy, Confocal	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Nanoparticles	en_US
dc.title	Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	7	en_US
utslib.for	0205 Optical Physics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	7	en_US

Abstract:

In this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. With synchronization-free STED, we imaged 200 nm nanospheres as well as all three cytoskeletal elements (microtubules, intermediate filaments, and actin filaments), clearly demonstrating the resolving power of synchronization-free STED over conventional diffraction limited imaging. It also allowed us to discover that, Dylight 650, exhibits improved performance over ATTO647N, a fluorophore frequently used in STED. Furthermore, we applied synchronization-free STED to image fluorescently-labeled intracellular viral RNA granules, which otherwise cannot be differentiated by confocal microscopy. Thanks to the widely available Ti:Sapphire oscillators in multiphoton imaging system, this work suggests easier access to setup super-resolution microscope via the synchronization-free STED. © 2012 Liu et al.

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http://hdl.handle.net/10453/37058