Rapid light curves: A powerful tool to assess photosynthetic activity

Ralph, PJ; Gademann, R

Rapid light curves: A powerful tool to assess photosynthetic activity

Ralph, PJ

Gademann, R

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Publication Type:: Journal Article
Citation:: Aquatic Botany, 2005, 82 (3), pp. 222 - 237
Issue Date:: 2005-07-01

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Field	Value	Language
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.contributor.author	Gademann, R	en_US
dc.date.issued	2005-07-01	en_US
dc.identifier.citation	Aquatic Botany, 2005, 82 (3), pp. 222 - 237	en_US
dc.identifier.issn	0304-3770	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3706
dc.description.abstract	Rapid light curves provide detailed information on the saturation characteristics of electron transport, as well as the overall photosynthetic performance of a plant. Rapid light curves were collected from samples of Zostera marina grown under low and high-light conditions (50 and 300 μmol photons m-2 s-1) and the distinctive patterns of RLC parameters are discussed, in terms of differential sink capacity and PSII reaction centre closure. Derived cardinal points of a rapid light curve (α, Ek and rETRmax) describe the photosynthetic capacity of a seagrass leaf, its light adaptation state and its capacity to tolerate short-term changes in light. The shapes of the corresponding F and F′m curves also provide information on the development of the trans-thylakoid proton gradient and thermal energy dissipation. Low-light leaves showed limited photosynthetic capacity and reduced activity of non-photochemical quenching pathways, whereas photosynthesis of high light leaves were not limited and showed an elevated level of non-photochemical quenching, possibly associated with xanthophyll cycle activity. Light-dark kinetics are also discussed in relation to relaxation of non-photochemical quenching and its various components. A curve fitting model is recommended based on the double exponential decay function. In this paper, we explain the fundamental aspects of a RLC, describe how it reflects the response to light exposure of a leaf, how to interpret these curves, and how to quantitatively describe and compare RLCs. © 2005 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Aquatic Botany	en_US
dc.relation.isbasedon	10.1016/j.aquabot.2005.02.006	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.title	Rapid light curves: A powerful tool to assess photosynthetic activity	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	82	en_US
utslib.for	060701 Phycology (incl. Marine Grasses)	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	0602 Ecology	en_US
utslib.for	0607 Plant Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	82	en_US

Abstract:

Rapid light curves provide detailed information on the saturation characteristics of electron transport, as well as the overall photosynthetic performance of a plant. Rapid light curves were collected from samples of Zostera marina grown under low and high-light conditions (50 and 300 μmol photons m-2 s-1) and the distinctive patterns of RLC parameters are discussed, in terms of differential sink capacity and PSII reaction centre closure. Derived cardinal points of a rapid light curve (α, Ek and rETRmax) describe the photosynthetic capacity of a seagrass leaf, its light adaptation state and its capacity to tolerate short-term changes in light. The shapes of the corresponding F and F′m curves also provide information on the development of the trans-thylakoid proton gradient and thermal energy dissipation. Low-light leaves showed limited photosynthetic capacity and reduced activity of non-photochemical quenching pathways, whereas photosynthesis of high light leaves were not limited and showed an elevated level of non-photochemical quenching, possibly associated with xanthophyll cycle activity. Light-dark kinetics are also discussed in relation to relaxation of non-photochemical quenching and its various components. A curve fitting model is recommended based on the double exponential decay function. In this paper, we explain the fundamental aspects of a RLC, describe how it reflects the response to light exposure of a leaf, how to interpret these curves, and how to quantitatively describe and compare RLCs. © 2005 Elsevier B.V. All rights reserved.

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http://hdl.handle.net/10453/3706