Toward autonomous measurements of photosynthetic electron transport rates: An evaluation of active fluorescence-based measurements of photochemistry

Silsbe, GM; Oxborough, K; Suggett, DJ; Forster, RM; Ihnken, S; Komárek, O; Lawrenz, E; Prášil, O; Röttgers, R; Šicner, M; Simis, SGH; Van Dijk, MA; Kromkamp, JC

Toward autonomous measurements of photosynthetic electron transport rates: An evaluation of active fluorescence-based measurements of photochemistry

Silsbe, GM Oxborough, K Suggett, DJ

Forster, RM Ihnken, S Komárek, O Lawrenz, E Prášil, O Röttgers, R Šicner, M Simis, SGH Van Dijk, MA Kromkamp, JC

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Publication Type:: Journal Article
Citation:: Limnology and Oceanography: Methods, 2015, 13 (3), pp. 138 - 155
Issue Date:: 2015-01-01

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Field	Value	Language
dc.contributor.author	Silsbe, GM	en_US
dc.contributor.author	Oxborough, K	en_US
dc.contributor.author	Suggett, DJ https://orcid.org/0000-0001-5326-2520	en_US
dc.contributor.author	Forster, RM	en_US
dc.contributor.author	Ihnken, S	en_US
dc.contributor.author	Komárek, O	en_US
dc.contributor.author	Lawrenz, E	en_US
dc.contributor.author	Prášil, O	en_US
dc.contributor.author	Röttgers, R	en_US
dc.contributor.author	Šicner, M	en_US
dc.contributor.author	Simis, SGH	en_US
dc.contributor.author	Van Dijk, MA	en_US
dc.contributor.author	Kromkamp, JC	en_US
dc.date.issued	2015-01-01	en_US
dc.identifier.citation	Limnology and Oceanography: Methods, 2015, 13 (3), pp. 138 - 155	en_US
dc.identifier.uri	http://hdl.handle.net/10453/37328
dc.description.abstract	© 2015 Association for the Sciences of Limnology and Oceanography. This study presents a methods evaluation and intercalibration of active fluorescence-based measurements of the quantum yield (ϕʹ 0 PSII) and absorption coefficient (aPSII) of photosystem II (PSII) photochemistry. Measurements of ϕʹPSII, aPSII, and irradiance (E) can be scaled to derive photosynthetic electron transport rates (Pe), the process that fuels phytoplankton carbon fixation and growth. Bio-optical estimates of ϕ PSII and aPSII were evaluated using 10 phytoplankton cultures across different pigment groups with varying bio-optical absorption characteristics on six different fast-repetition rate fluorometers that span two different manufacturers and four different models. Culture measurements of ϕʹ PSII and the effective absorption cross section of PSII photochemistry (σPSII, a constituent of aPSII) showed a high degree of correspondence across instruments, although some instrument-specific biases are identified. A range of approaches have been used in the literature to estimate aPSII(λ) and are evaluated here.With the exception of ex situ aPSII(λ) estimates from paired σPSII and PSII reaction center concentration ([RCII]) measurements, the accuracy and precision of in situ aPSII(λ) methodologies are largely determined by the variance of method-specific coefficients. The accuracy and precision of these coefficients are evaluated, compared to literature data, and discussed within a framework of autonomous Pe measurements. This study supports the application of an instrument-specific calibration coefficient (KR) that scales minimum fluorescence in the dark (F0) to aPSII as both the most accurate in situ measurement of aPSII, and the methodology best suited for highly resolved autonomous Pe measurements.VC 2014 Association for the Sciences of Limnology and Oceanography.	en_US
dc.relation.ispartof	Limnology and Oceanography: Methods	en_US
dc.relation.isbasedon	10.1002/lom3.10014	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.title	Toward autonomous measurements of photosynthetic electron transport rates: An evaluation of active fluorescence-based measurements of photochemistry	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	13	en_US
utslib.for	060705 Plant Physiology	en_US
utslib.for	060701 Phycology (incl. Marine Grasses)	en_US
utslib.for	04 Earth Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	13	en_US

Abstract:

© 2015 Association for the Sciences of Limnology and Oceanography. This study presents a methods evaluation and intercalibration of active fluorescence-based measurements of the quantum yield (ϕʹ 0 PSII) and absorption coefficient (aPSII) of photosystem II (PSII) photochemistry. Measurements of ϕʹPSII, aPSII, and irradiance (E) can be scaled to derive photosynthetic electron transport rates (Pe), the process that fuels phytoplankton carbon fixation and growth. Bio-optical estimates of ϕ PSII and aPSII were evaluated using 10 phytoplankton cultures across different pigment groups with varying bio-optical absorption characteristics on six different fast-repetition rate fluorometers that span two different manufacturers and four different models. Culture measurements of ϕʹ PSII and the effective absorption cross section of PSII photochemistry (σPSII, a constituent of aPSII) showed a high degree of correspondence across instruments, although some instrument-specific biases are identified. A range of approaches have been used in the literature to estimate aPSII(λ) and are evaluated here.With the exception of ex situ aPSII(λ) estimates from paired σPSII and PSII reaction center concentration ([RCII]) measurements, the accuracy and precision of in situ aPSII(λ) methodologies are largely determined by the variance of method-specific coefficients. The accuracy and precision of these coefficients are evaluated, compared to literature data, and discussed within a framework of autonomous Pe measurements. This study supports the application of an instrument-specific calibration coefficient (KR) that scales minimum fluorescence in the dark (F0) to aPSII as both the most accurate in situ measurement of aPSII, and the methodology best suited for highly resolved autonomous Pe measurements.VC 2014 Association for the Sciences of Limnology and Oceanography.

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