Identification of vascular surface proteins by in vivo biotinylation: A method sufficiently sensitive to detect changes in rat liver 2 weeks after partial hepatectomy

McCann, LA; Haywood, MC; Bin, HR; Simpson, AM; Guilhaus, M; Wasinger, VC; Raftery, MJ; Davey, RA

Identification of vascular surface proteins by in vivo biotinylation: A method sufficiently sensitive to detect changes in rat liver 2 weeks after partial hepatectomy

McCann, LA Haywood, MC Bin, HR Simpson, AM

Guilhaus, M Wasinger, VC Raftery, MJ Davey, RA

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Publication Type:: Journal Article
Citation:: Journal of Proteome Research, 2007, 6 (8), pp. 3108 - 3113
Issue Date:: 2007-08-01

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Field	Value	Language
dc.contributor.author	McCann, LA	en_US
dc.contributor.author	Haywood, MC	en_US
dc.contributor.author	Bin, HR	en_US
dc.contributor.author	Simpson, AM https://orcid.org/0000-0002-2249-5097	en_US
dc.contributor.author	Guilhaus, M	en_US
dc.contributor.author	Wasinger, VC	en_US
dc.contributor.author	Raftery, MJ	en_US
dc.contributor.author	Davey, RA	en_US
dc.date.issued	2007-08-01	en_US
dc.identifier.citation	Journal of Proteome Research, 2007, 6 (8), pp. 3108 - 3113	en_US
dc.identifier.issn	1535-3893	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3759
dc.description.abstract	We have developed a methodology to selectively isolate and identify proteins associated with the luminal surface of blood vessels using in vivo biotinylation, streptavidin-affinity chromatography, and SDS-PAGE/LC-MS/MS. This had sufficient sensitivity to identify 32 proteins with changed expression in rat livers at 2 weeks or 5 weeks after partial hepatectomy, well after the 7 day tissue remodeling period. This method could be adapted to study other angiogenic tissues including tumors. © 2007 American Chemical Society.	en_US
dc.relation.ispartof	Journal of Proteome Research	en_US
dc.relation.isbasedon	10.1021/pr070032m	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Liver	en_US
dc.subject.mesh	Blood Vessels	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Rats, Inbred F344	en_US
dc.subject.mesh	Rats	en_US
dc.subject.mesh	Membrane Proteins	en_US
dc.subject.mesh	Proteome	en_US
dc.subject.mesh	Hepatectomy	en_US
dc.subject.mesh	Chromatography, Liquid	en_US
dc.subject.mesh	Chromatography, Affinity	en_US
dc.subject.mesh	Electrophoresis, Gel, Two-Dimensional	en_US
dc.subject.mesh	Biotinylation	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.title	Identification of vascular surface proteins by in vivo biotinylation: A method sufficiently sensitive to detect changes in rat liver 2 weeks after partial hepatectomy	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	6	en_US
utslib.for	0699 Other Biological Sciences	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	06 Biological Sciences	en_US
dc.location.activity	ISI:000248683200018	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	6	en_US

Abstract:

We have developed a methodology to selectively isolate and identify proteins associated with the luminal surface of blood vessels using in vivo biotinylation, streptavidin-affinity chromatography, and SDS-PAGE/LC-MS/MS. This had sufficient sensitivity to identify 32 proteins with changed expression in rat livers at 2 weeks or 5 weeks after partial hepatectomy, well after the 7 day tissue remodeling period. This method could be adapted to study other angiogenic tissues including tumors. © 2007 American Chemical Society.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/3759