Identification of vascular surface proteins by in vivo biotinylation: A method sufficiently sensitive to detect changes in rat liver 2 weeks after partial hepatectomy
- Publication Type:
- Journal Article
- Journal of Proteome Research, 2007, 6 (8), pp. 3108 - 3113
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We have developed a methodology to selectively isolate and identify proteins associated with the luminal surface of blood vessels using in vivo biotinylation, streptavidin-affinity chromatography, and SDS-PAGE/LC-MS/MS. This had sufficient sensitivity to identify 32 proteins with changed expression in rat livers at 2 weeks or 5 weeks after partial hepatectomy, well after the 7 day tissue remodeling period. This method could be adapted to study other angiogenic tissues including tumors. © 2007 American Chemical Society.
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