Evaluation of cadmium genotoxicity in Lactuca sativa L. using nuclear microsatellites

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Journal Article
Environmental and Experimental Botany, 2007, 60 (3), pp. 421 - 427
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Cadmium (Cd) is a non-essential element and is a widespread environmental pollutant. Exposure to cadmium can resultin cytoxic, carcinogenic and mytagenic effects. Mutagenesis is indicative of genetic instability and can be assayed using microsatellites. Microsatellites or simple sequence repeats (SSRs) are composed of tandem repeats of short sequence motifs (1-6 bp) that are polymorphic, mainly in the number of tandem repeated units. Therefore, chromosomic mutations like inversions, deletion or translocation and point mutations can be detected by this type of molecular marker. In this study we have evaluated the mutagenic/genotoxic effects of cadmium in leetuce (Lactuca sativa L). Five-week old lettuce plants grown in a modified Hoagland's medium were exposed for a further 14 days to a demium containing 100 uMCd (NO subscript3)subscript2. Genomic DNA was extracted from lettuce leaves and roots harvested at days 0, 1, 3, 7 and 14 and nince SSRs were tested, amplified and analysed to evaluate microsatellite intsability (MSI). Mutagenic effects of cadmium on microsatellite DNA loci were assessed and no MSI wa sobserved in the used markers.
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