Proteomic and genomic analyses suggest the association of Apolipoprotein C1 with abdominal aortic aneurysm.

Padula, M

Proteomic and genomic analyses suggest the association of Apolipoprotein C1 with abdominal aortic aneurysm.

Padula, M

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Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Proteomics - Clinical Applications, 2014, 8 (9-10), pp. 762 - 772
Issue Date:: 2014-01

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Field	Value	Language
dc.contributor.author	Padula, M	en_US
dc.date.issued	2014-01	en_US
dc.identifier.citation	Proteomics - Clinical Applications, 2014, 8 (9-10), pp. 762 - 772	en_US
dc.identifier.issn	1862-8354	en_US
dc.identifier.uri	http://hdl.handle.net/10453/37791
dc.description.abstract	Purpose Abdominal aortic aneurysm (AAA) is an important cause of mortality in the elderly. Mouse models are widely used to investigate AAA pathogenesis but their suitability for biomarker discovery is unexplored. Experimental design We conducted a three-phase study. Phase 1: Aortas from angiotensin-II-infused apolipoprotein E deficient (ApoE−/−) mice with and without AAA were assessed via iTRAQ and analyzed in silico to identify potential circulating markers. Microarray data from ApoE−/− mice and human patients were analyzed in parallel. Phase 2: Putative markers were compared between datasets to shortlist common candidates. Phase 3: The relationship of two shortlisted markers and AAA presence was assessed. Results iTRAQ identified eight proteins with biomarker potential. Microarray data identified 72 and 96 potential biomarkers from ApoE−/− mice and human patients, respectively. All three datasets suggested apolipoprotein C1 (ApoC1) as a marker for AAA; microarray data identified matrix metalloproteinase 9 (MMP9) as a second potential marker. Plasma ApoC1 and MMP9 concentrations positively correlated with AAA diameter in ApoE−/− mice.	en_US
dc.publisher	Wiley	en_US
dc.relation.ispartof	Proteomics - Clinical Applications	en_US
dc.relation.isbasedon	10.1002/prca.201300119	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.title	Proteomic and genomic analyses suggest the association of Apolipoprotein C1 with abdominal aortic aneurysm.	en_US
dc.type	Journal Article
utslib.citation.volume	9-10	en_US
utslib.citation.volume	8	en_US
utslib.for	1101 Medical Biochemistry And Metabolomics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.declined	1970-01-01T00:00:00.0+1000
pubs.consider-herdc	true	en_US
pubs.deleted	1970-01-01T00:00:00.0+1000
pubs.issue	9-10	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

Purpose Abdominal aortic aneurysm (AAA) is an important cause of mortality in the elderly. Mouse models are widely used to investigate AAA pathogenesis but their suitability for biomarker discovery is unexplored. Experimental design We conducted a three-phase study. Phase 1: Aortas from angiotensin-II-infused apolipoprotein E deficient (ApoE−/−) mice with and without AAA were assessed via iTRAQ and analyzed in silico to identify potential circulating markers. Microarray data from ApoE−/− mice and human patients were analyzed in parallel. Phase 2: Putative markers were compared between datasets to shortlist common candidates. Phase 3: The relationship of two shortlisted markers and AAA presence was assessed. Results iTRAQ identified eight proteins with biomarker potential. Microarray data identified 72 and 96 potential biomarkers from ApoE−/− mice and human patients, respectively. All three datasets suggested apolipoprotein C1 (ApoC1) as a marker for AAA; microarray data identified matrix metalloproteinase 9 (MMP9) as a second potential marker. Plasma ApoC1 and MMP9 concentrations positively correlated with AAA diameter in ApoE−/− mice.

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http://hdl.handle.net/10453/37791