Broad spectrum antimicrobial activity of melimine covalently bound to contact lenses

Dutta, D; Cole, N; Kumar, N; Willcox, MDP

Broad spectrum antimicrobial activity of melimine covalently bound to contact lenses

Dutta, D Cole, N

Kumar, N Willcox, MDP

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Publication Type:: Journal Article
Citation:: Investigative Ophthalmology and Visual Science, 2013, 54 (1), pp. 175 - 182
Issue Date:: 2013-01-01

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Field	Value	Language
dc.contributor.author	Dutta, D	en_US
dc.contributor.author	Cole, N https://orcid.org/0000-0002-9866-4694	en_US
dc.contributor.author	Kumar, N	en_US
dc.contributor.author	Willcox, MDP	en_US
dc.date.issued	2013-01-01	en_US
dc.identifier.citation	Investigative Ophthalmology and Visual Science, 2013, 54 (1), pp. 175 - 182	en_US
dc.identifier.issn	0146-0404	en_US
dc.identifier.uri	http://hdl.handle.net/10453/37803
dc.description.abstract	PURPOSE. To develop a stable antimicrobial contact lens, which is effective against the International Organization for Standardization (ISO) panel microorganisms, Acanthamoeba castellanii and drug resistant strains of Pseudomonas aeruginosa and Staphylococcus aureus. METHODS. Melimine was covalently incorporated into etafilcon A lenses. The amount of peptide present on the lens surface was quantified using amino acid analysis. After coating, the heat stability (121°C), lens surface hydrophobicity (by captive bubble), and in vitro cytotoxicity to mouse L929 cells of the lenses were investigated. Antimicrobial activity against the micro-organisms was evaluated by viable plate count and fluorescence microscopy, measuring the proportion of cell death compared with control lenses with no melimine. RESULTS. The most effective concentration was determined to be 152 ± 44 lg lens-1 melimine on the lens surface. After coating, lenses were relatively hydrophilic and were nontoxic to mammalian cells. The activity remained high after autoclaving (e.g., 3.1, 3.9, 1.2, and 1.0 log inhibition against P. aeruginosa, S. aureus, A. castellanii, and Fusarium solani, respectively). Fluorescence microscopy confirmed significantly reduced (P < 0.001) adhesion of viable bacteria to melimine contact lenses. Viable count confirmed that lenses were active against all the bacteria and fungi from the ISO panel, Acanthamoeba and gave at least 2 log inhibition against all the multidrug resistant S. aureus and P. aeruginosa strains. CONCLUSIONS. Melimine may offer excellent potential for development as a broad spectrum antimicrobial coating for contact lenses, showing activity against all the bacterial and fungal ISO panel microorganisms, Acanthamoeba, and antibiotic resistant strains of P. aeruginosa and S. aureus. © 2013 The Association for Research in Vision and Ophthalmology, Inc.	en_US
dc.relation.ispartof	Investigative Ophthalmology and Visual Science	en_US
dc.relation.isbasedon	10.1167/iovs.12-10989	en_US
dc.subject.classification	Ophthalmology & Optometry	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Acanthamoeba castellanii	en_US
dc.subject.mesh	Eye Infections, Bacterial	en_US
dc.subject.mesh	Pseudomonas Infections	en_US
dc.subject.mesh	Staphylococcal Infections	en_US
dc.subject.mesh	Amebiasis	en_US
dc.subject.mesh	Fibrosarcoma	en_US
dc.subject.mesh	Keratitis	en_US
dc.subject.mesh	Methacrylates	en_US
dc.subject.mesh	Antimicrobial Cationic Peptides	en_US
dc.subject.mesh	Coated Materials, Biocompatible	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Materials Testing	en_US
dc.subject.mesh	Contact Lenses	en_US
dc.subject.mesh	Drug Resistance, Bacterial	en_US
dc.subject.mesh	Hydrophobic and Hydrophilic Interactions	en_US
dc.subject.mesh	Fusariosis	en_US
dc.title	Broad spectrum antimicrobial activity of melimine covalently bound to contact lenses	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	54	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	54	en_US

Abstract:

PURPOSE. To develop a stable antimicrobial contact lens, which is effective against the International Organization for Standardization (ISO) panel microorganisms, Acanthamoeba castellanii and drug resistant strains of Pseudomonas aeruginosa and Staphylococcus aureus. METHODS. Melimine was covalently incorporated into etafilcon A lenses. The amount of peptide present on the lens surface was quantified using amino acid analysis. After coating, the heat stability (121°C), lens surface hydrophobicity (by captive bubble), and in vitro cytotoxicity to mouse L929 cells of the lenses were investigated. Antimicrobial activity against the micro-organisms was evaluated by viable plate count and fluorescence microscopy, measuring the proportion of cell death compared with control lenses with no melimine. RESULTS. The most effective concentration was determined to be 152 ± 44 lg lens-1 melimine on the lens surface. After coating, lenses were relatively hydrophilic and were nontoxic to mammalian cells. The activity remained high after autoclaving (e.g., 3.1, 3.9, 1.2, and 1.0 log inhibition against P. aeruginosa, S. aureus, A. castellanii, and Fusarium solani, respectively). Fluorescence microscopy confirmed significantly reduced (P < 0.001) adhesion of viable bacteria to melimine contact lenses. Viable count confirmed that lenses were active against all the bacteria and fungi from the ISO panel, Acanthamoeba and gave at least 2 log inhibition against all the multidrug resistant S. aureus and P. aeruginosa strains. CONCLUSIONS. Melimine may offer excellent potential for development as a broad spectrum antimicrobial coating for contact lenses, showing activity against all the bacterial and fungal ISO panel microorganisms, Acanthamoeba, and antibiotic resistant strains of P. aeruginosa and S. aureus. © 2013 The Association for Research in Vision and Ophthalmology, Inc.

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http://hdl.handle.net/10453/37803