Response of an arctic sediment nitrogen cycling community to increased CO<inf>2</inf>

Tait, K; Laverock, B; Widdicombe, S

Response of an arctic sediment nitrogen cycling community to increased CO<inf>2</inf>

Tait, K Laverock, B Widdicombe, S

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Publication Type:: Journal Article
Citation:: Estuaries and Coasts, 2014, 37 (3), pp. 724 - 735
Issue Date:: 2014-01-01

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Field	Value	Language
dc.contributor.author	Tait, K	en_US
dc.contributor.author	Laverock, B	en_US
dc.contributor.author	Widdicombe, S	en_US
dc.date.issued	2014-01-01	en_US
dc.identifier.citation	Estuaries and Coasts, 2014, 37 (3), pp. 724 - 735	en_US
dc.identifier.issn	1559-2723	en_US
dc.identifier.uri	http://hdl.handle.net/10453/37818
dc.description.abstract	© Coastal and Estuarine Research Federation 2013. Ocean acidification influences sediment/water nitrogen fluxes, possibly by impacting on the microbial process of ammonia oxidation. To investigate this further, undisturbed sediment cores collected from Ny Alesund harbour (Svalbard) were incubated with seawater adjusted to CO2concentrations of 380, 540, 760, 1,120 and 3,000 μatm. DNA and RNAwere extracted from the sediment surface after 14 days' exposure and the abundance of bacterial and archaeal ammonia oxidising (amoA) genes and transcripts quantified using quantitative polymerase chain reaction. While there was no change to the abundance of bacterial amoA genes, an increase to 760 μatm pCO2reduced the abundance of bacterial amoA transcripts by 65 %, and this was accompanied by a shift in the composition of the active community. In contrast, archaeal amoA gene and transcript abundance both doubled at 3,000 μatm, with an increase in species richness also apparent. This suggests that ammonia oxidising bacteria and archaea in marine sediments have different pH optima, and the impact of elevated CO2on N cycling may be dependent on the relative abundances of these two major microbial groups. Further evidence of a shift in the balance of key N cycling groups was also evident: the abundance of nirS-type denitrifier transcripts decreased alongside bacterial amoA transcripts, indicating that NO3−produced by bacterial nitrification fuelled denitrification. An increase in the abundance of Planctomycete-specific 16S rRNA, the vastmajority of which grouped with known anammox bacteria, was also apparent at 3,000 μatm pCO2. This could indicate a possible shift from coupled nitrification-denitrification to anammox activity at elevated CO2.	en_US
dc.relation.ispartof	Estuaries and Coasts	en_US
dc.relation.isbasedon	10.1007/s12237-013-9709-x	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.title	Response of an arctic sediment nitrogen cycling community to increased CO<inf>2</inf>	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	37	en_US
utslib.for	04 Earth Sciences	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	37	en_US

Abstract:

© Coastal and Estuarine Research Federation 2013. Ocean acidification influences sediment/water nitrogen fluxes, possibly by impacting on the microbial process of ammonia oxidation. To investigate this further, undisturbed sediment cores collected from Ny Alesund harbour (Svalbard) were incubated with seawater adjusted to CO2concentrations of 380, 540, 760, 1,120 and 3,000 μatm. DNA and RNAwere extracted from the sediment surface after 14 days' exposure and the abundance of bacterial and archaeal ammonia oxidising (amoA) genes and transcripts quantified using quantitative polymerase chain reaction. While there was no change to the abundance of bacterial amoA genes, an increase to 760 μatm pCO2reduced the abundance of bacterial amoA transcripts by 65 %, and this was accompanied by a shift in the composition of the active community. In contrast, archaeal amoA gene and transcript abundance both doubled at 3,000 μatm, with an increase in species richness also apparent. This suggests that ammonia oxidising bacteria and archaea in marine sediments have different pH optima, and the impact of elevated CO2on N cycling may be dependent on the relative abundances of these two major microbial groups. Further evidence of a shift in the balance of key N cycling groups was also evident: the abundance of nirS-type denitrifier transcripts decreased alongside bacterial amoA transcripts, indicating that NO3−produced by bacterial nitrification fuelled denitrification. An increase in the abundance of Planctomycete-specific 16S rRNA, the vastmajority of which grouped with known anammox bacteria, was also apparent at 3,000 μatm pCO2. This could indicate a possible shift from coupled nitrification-denitrification to anammox activity at elevated CO2.

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http://hdl.handle.net/10453/37818