Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation

Publication Type:
Journal Article
Citation:
FEMS Microbiology Letters, 2005, 247 (1), pp. 73 - 79
Issue Date:
2005-06-01
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It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (β subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (τ subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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