Bacillus subtilis YabA is involved in determining the timing and synchrony of replication initiation
- Publication Type:
- Journal Article
- FEMS Microbiology Letters, 2005, 247 (1), pp. 73 - 79
- Issue Date:
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (β subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (τ subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Please use this identifier to cite or link to this item: