Requirement for the cell division protein DivIB in polar cell division and engulfment during sporulation in Bacillus subtilis

Thompson, LS; Beech, PL; Real, G; Henriques, AO; Harry, EJ

Requirement for the cell division protein DivIB in polar cell division and engulfment during sporulation in Bacillus subtilis

Thompson, LS Beech, PL Real, G Henriques, AO Harry, EJ

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Publication Type:: Journal Article
Citation:: Journal of Bacteriology, 2006, 188 (21), pp. 7677 - 7685
Issue Date:: 2006-11-01

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Field	Value	Language
dc.contributor.author	Thompson, LS	en_US
dc.contributor.author	Beech, PL	en_US
dc.contributor.author	Real, G	en_US
dc.contributor.author	Henriques, AO	en_US
dc.contributor.author	Harry, EJ https://orcid.org/0000-0003-0858-9473	en_US
dc.date.issued	2006-11-01	en_US
dc.identifier.citation	Journal of Bacteriology, 2006, 188 (21), pp. 7677 - 7685	en_US
dc.identifier.issn	0021-9193	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3862
dc.description.abstract	During spore formation in Bacillus subtilis, cell division occurs at the cell pole and is believed to require essentially the same division machinery as vegetative division. Intriguingly, although the cell division protein DivIB is not required for vegetative division at low temperatures, it is essential for efficient sporulation under these conditions. We show here that at low temperatures in the absence of DivIB, formation of the polar septum during sporulation is delayed and less efficient. Furthermore, the polar septa that are complete are abnormally thick, containing more peptidoglycan than a normal polar septum. These results show that DivIB is specifically required for the efficient and correct formation of a polar septum. This suggests that DivIB is required for the modification of sporulation septal peptidoglycan, raising the possibility that DivIB either regulates hydrolysis of polar septal peptidoglycan or is a hydrolase itself. We also show that, despite the significant number of completed polar septa that form in this mutant, it is unable to undergo engulfment. Instead, hydrolysis of the peptidoglycan within the polar septum, which occurs during the early stages of engulfment, is incomplete, producing a similar phenotype to that of mutants defective in the production of sporulation-specific septal peptidoglycan hydrolases. We propose a role for DivIB in sporulation-specific peptidoglycan remodelling or its regulation during polar septation and engulfment. Copyright © 2006, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DP0450770
dc.relation.ispartof	Journal of Bacteriology	en_US
dc.relation.isbasedon	10.1128/JB.01072-06	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Cell Wall	en_US
dc.subject.mesh	Bacillus subtilis	en_US
dc.subject.mesh	Spores, Bacterial	en_US
dc.subject.mesh	N-Acetylmuramoyl-L-alanine Amidase	en_US
dc.subject.mesh	Peptidoglycan	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Green Fluorescent Proteins	en_US
dc.subject.mesh	Membrane Proteins	en_US
dc.subject.mesh	Microscopy, Electron, Transmission	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Staining and Labeling	en_US
dc.subject.mesh	Temperature	en_US
dc.subject.mesh	Cell Division	en_US
dc.subject.mesh	Genes, Reporter	en_US
dc.title	Requirement for the cell division protein DivIB in polar cell division and engulfment during sporulation in Bacillus subtilis	en_US
dc.type	Journal Article
utslib.citation.volume	21	en_US
utslib.citation.volume	188	en_US
utslib.for	060503 Microbial Genetics	en_US
utslib.for	060501 Bacteriology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	21	en_US
pubs.publication-status	Published	en_US
pubs.volume	188	en_US

Abstract:

During spore formation in Bacillus subtilis, cell division occurs at the cell pole and is believed to require essentially the same division machinery as vegetative division. Intriguingly, although the cell division protein DivIB is not required for vegetative division at low temperatures, it is essential for efficient sporulation under these conditions. We show here that at low temperatures in the absence of DivIB, formation of the polar septum during sporulation is delayed and less efficient. Furthermore, the polar septa that are complete are abnormally thick, containing more peptidoglycan than a normal polar septum. These results show that DivIB is specifically required for the efficient and correct formation of a polar septum. This suggests that DivIB is required for the modification of sporulation septal peptidoglycan, raising the possibility that DivIB either regulates hydrolysis of polar septal peptidoglycan or is a hydrolase itself. We also show that, despite the significant number of completed polar septa that form in this mutant, it is unable to undergo engulfment. Instead, hydrolysis of the peptidoglycan within the polar septum, which occurs during the early stages of engulfment, is incomplete, producing a similar phenotype to that of mutants defective in the production of sporulation-specific septal peptidoglycan hydrolases. We propose a role for DivIB in sporulation-specific peptidoglycan remodelling or its regulation during polar septation and engulfment. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/3862