Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes.

Kong, F; Brown, M; Sabananthan, A; Zeng, X; Gilbert, GL

Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes.

Kong, F Brown, M Sabananthan, A Zeng, X Gilbert, GL

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Publication Type:: Journal Article
Citation:: J Clin Microbiol, 2006, 44 (5), pp. 1887 - 1891
Issue Date:: 2006-05

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Field	Value	Language
dc.contributor.author	Kong, F	en_US
dc.contributor.author	Brown, M	en_US
dc.contributor.author	Sabananthan, A	en_US
dc.contributor.author	Zeng, X	en_US
dc.contributor.author	Gilbert, GL	en_US
dc.date.issued	2006-05	en_US
dc.identifier.citation	J Clin Microbiol, 2006, 44 (5), pp. 1887 - 1891	en_US
dc.identifier.issn	0095-1137	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3869
dc.description.abstract	We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-react with 1 to 4 nonvaccine serotypes, could be distinguished using serotype-specific antisera.	en_US
dc.language	eng	en_US
dc.relation.ispartof	J Clin Microbiol	en_US
dc.relation.isbasedon	10.1128/JCM.44.5.1887-1891.2006	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Streptococcus pneumoniae	en_US
dc.subject.mesh	Polysaccharides, Bacterial	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	DNA Probes	en_US
dc.subject.mesh	DNA Primers	en_US
dc.subject.mesh	Pneumococcal Vaccines	en_US
dc.subject.mesh	Antibodies, Bacterial	en_US
dc.subject.mesh	Serotyping	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Cross Reactions	en_US
dc.subject.mesh	Base Sequence	en_US
dc.title	Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes.	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	44	en_US
utslib.location.activity	United States	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	Yokohama, Japan
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	44	en_US

Abstract:

We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-react with 1 to 4 nonvaccine serotypes, could be distinguished using serotype-specific antisera.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/3869