Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes.

Publication Type:
Journal Article
Citation:
J Clin Microbiol, 2006, 44 (5), pp. 1887 - 1891
Issue Date:
2006-05
Filename Description Size
Thumbnail2006004002.pdf412.81 kB
Adobe PDF
Full metadata record
We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-react with 1 to 4 nonvaccine serotypes, could be distinguished using serotype-specific antisera.
Please use this identifier to cite or link to this item: