Detection of Dientamoeba fragilis in fresh stool specimens using PCR

Stark, D; Beebe, N; Marriott, D; Ellis, J; Harkness, J

Detection of Dientamoeba fragilis in fresh stool specimens using PCR

Stark, D Beebe, N

Marriott, D Ellis, J

Harkness, J

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Publication Type:: Journal Article
Citation:: International Journal for Parasitology, 2005, 35 (1), pp. 57 - 62
Issue Date:: 2005-01-01

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Field	Value	Language
dc.contributor.author	Stark, D	en_US
dc.contributor.author	Beebe, N https://orcid.org/0000-0003-2877-0691	en_US
dc.contributor.author	Marriott, D	en_US
dc.contributor.author	Ellis, J https://orcid.org/0000-0001-7328-4831	en_US
dc.contributor.author	Harkness, J	en_US
dc.date.available	2004-09-14	en_US
dc.date.issued	2005-01-01	en_US
dc.identifier.citation	International Journal for Parasitology, 2005, 35 (1), pp. 57 - 62	en_US
dc.identifier.issn	0020-7519	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3883
dc.description.abstract	Dientamoeba fragilis is a trichomonad parasite that causes human gastrointestinal disease. Currently microscopy is considered to be the gold standard for diagnosis of D. fragilis infection. However, this method is time-consuming and relatively insensitive. A PCR assay based on the small-subunit ribosomal RNA gene of D. fragilis for the specific detection of D. fragilis DNA in fresh unpreserved stool samples was developed. The D. fragilis PCR was positive in 29/31 samples with positive microscopy and did not cross-react with other protozoan parasites. The PCR protocol showed a specificity of 100% and a sensitivity of 93.5% and the entire procedure can be performed in one day. © 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.	en_US
dc.relation.ispartof	International Journal for Parasitology	en_US
dc.relation.isbasedon	10.1016/j.ijpara.2004.09.003	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Feces	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Dientamoeba	en_US
dc.subject.mesh	Dientamoebiasis	en_US
dc.subject.mesh	Diarrhea	en_US
dc.subject.mesh	DNA, Protozoan	en_US
dc.subject.mesh	Diagnosis, Differential	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Genes, Protozoan	en_US
dc.subject.mesh	Genes, rRNA	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Detection of Dientamoeba fragilis in fresh stool specimens using PCR	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	35	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	35	en_US

Abstract:

Dientamoeba fragilis is a trichomonad parasite that causes human gastrointestinal disease. Currently microscopy is considered to be the gold standard for diagnosis of D. fragilis infection. However, this method is time-consuming and relatively insensitive. A PCR assay based on the small-subunit ribosomal RNA gene of D. fragilis for the specific detection of D. fragilis DNA in fresh unpreserved stool samples was developed. The D. fragilis PCR was positive in 29/31 samples with positive microscopy and did not cross-react with other protozoan parasites. The PCR protocol showed a specificity of 100% and a sensitivity of 93.5% and the entire procedure can be performed in one day. © 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/3883