Detection of Dientamoeba fragilis in fresh stool specimens using PCR

Publication Type:
Journal Article
International Journal for Parasitology, 2005, 35 (1), pp. 57 - 62
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Dientamoeba fragilis is a trichomonad parasite that causes human gastrointestinal disease. Currently microscopy is considered to be the gold standard for diagnosis of D. fragilis infection. However, this method is time-consuming and relatively insensitive. A PCR assay based on the small-subunit ribosomal RNA gene of D. fragilis for the specific detection of D. fragilis DNA in fresh unpreserved stool samples was developed. The D. fragilis PCR was positive in 29/31 samples with positive microscopy and did not cross-react with other protozoan parasites. The PCR protocol showed a specificity of 100% and a sensitivity of 93.5% and the entire procedure can be performed in one day. © 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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