An inhibitory substance produced by Aeromonas media A199, an aquatic probiotic

Lategan, MJ; Booth, W; Shimmon, R; Gibson, LF

An inhibitory substance produced by Aeromonas media A199, an aquatic probiotic

Lategan, MJ Booth, W Shimmon, R

Gibson, LF

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Publication Type:: Journal Article
Citation:: Aquaculture, 2006, 254 (1-4), pp. 115 - 124
Issue Date:: 2006-04-28

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Field	Value	Language
dc.contributor.author	Lategan, MJ	en_US
dc.contributor.author	Booth, W	en_US
dc.contributor.author	Shimmon, R https://orcid.org/0000-0002-8763-991X	en_US
dc.contributor.author	Gibson, LF	en_US
dc.date.issued	2006-04-28	en_US
dc.identifier.citation	Aquaculture, 2006, 254 (1-4), pp. 115 - 124	en_US
dc.identifier.issn	0044-8486	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3901
dc.description.abstract	Whether or not the probiotic activity of the Aeromonas media strain A199 derived from the production of an extracellular inhibitory substance was investigated. Ethyl acetate extraction of broth cultures of A199 and preparative thin layer chromatography methodologies revealed a fraction that contained inhibitory activity against bacterial and fungal indicators. Gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) analyses identified indole (2,3 benzopyrrole, henceforth referred to as T1) as the major chemical component in this fraction. The presence of inhibitory activity in broth culture extracts of A199 was found to be entirely dependent on the production of T1 by the organism. The inhibitory activity of T1 in vitro against Edwardsiella tarda, Vibrio anguillarum, Yersinia ruckeri, Aeromonas salmonicida and Lactococcus garvieae was found to be concentration dependent (300-600 μg ml- 1). Antifungal activity (75-300 μg ml- 1) was obtained against the vegetative stage and cysts of Saprolegnia parasitica, with cysts showing a higher susceptibility. Morphological changes observed within hyphae suggested that T1 could be a potential cytoplasmic toxin. Equivalent inhibitory activity was obtained from commercial indole against the majority of indicators, but discrepancies were encountered consistently with failure to inhibit particular bacterial indicators as well as decreased antifungal activity. A comparison of the inhibitory activity of A199 and other indole producers, that included various strains of A. media and Escherichia coli, indicated that the ability of a bacterium to produce indole might not necessarily afford it with inhibitory activities. © 2005 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Aquaculture	en_US
dc.relation.isbasedon	10.1016/j.aquaculture.2005.11.019	en_US
dc.subject.classification	Fisheries	en_US
dc.title	An inhibitory substance produced by Aeromonas media A199, an aquatic probiotic	en_US
dc.type	Journal Article
utslib.citation.volume	1-4	en_US
utslib.citation.volume	254	en_US
utslib.for	0704 Fisheries Sciences	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CCET - Centre for Clean Energy Technology
utslib.copyright.status	closed_access
pubs.issue	1-4	en_US
pubs.publication-status	Published	en_US
pubs.volume	254	en_US

Abstract:

Whether or not the probiotic activity of the Aeromonas media strain A199 derived from the production of an extracellular inhibitory substance was investigated. Ethyl acetate extraction of broth cultures of A199 and preparative thin layer chromatography methodologies revealed a fraction that contained inhibitory activity against bacterial and fungal indicators. Gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) analyses identified indole (2,3 benzopyrrole, henceforth referred to as T1) as the major chemical component in this fraction. The presence of inhibitory activity in broth culture extracts of A199 was found to be entirely dependent on the production of T1 by the organism. The inhibitory activity of T1 in vitro against Edwardsiella tarda, Vibrio anguillarum, Yersinia ruckeri, Aeromonas salmonicida and Lactococcus garvieae was found to be concentration dependent (300-600 μg ml- 1). Antifungal activity (75-300 μg ml- 1) was obtained against the vegetative stage and cysts of Saprolegnia parasitica, with cysts showing a higher susceptibility. Morphological changes observed within hyphae suggested that T1 could be a potential cytoplasmic toxin. Equivalent inhibitory activity was obtained from commercial indole against the majority of indicators, but discrepancies were encountered consistently with failure to inhibit particular bacterial indicators as well as decreased antifungal activity. A comparison of the inhibitory activity of A199 and other indole producers, that included various strains of A. media and Escherichia coli, indicated that the ability of a bacterium to produce indole might not necessarily afford it with inhibitory activities. © 2005 Elsevier B.V. All rights reserved.

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http://hdl.handle.net/10453/3901