The development of the macrogamete and oocyst wall in Eimeria maxima: Immuno-light and electron microscopy

Ferguson, DJP; Belli, SI; Smith, NC; Wallach, MG

The development of the macrogamete and oocyst wall in Eimeria maxima: Immuno-light and electron microscopy

Ferguson, DJP Belli, SI

Smith, NC

Wallach, MG

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Publication Type:: Journal Article
Citation:: International Journal for Parasitology, 2003, 33 (12), pp. 1329 - 1340
Issue Date:: 2003-01-01

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Field	Value	Language
dc.contributor.author	Ferguson, DJP	en_US
dc.contributor.author	Belli, SI https://orcid.org/0000-0003-3845-326X	en_US
dc.contributor.author	Smith, NC https://orcid.org/0000-0002-7467-1451	en_US
dc.contributor.author	Wallach, MG	en_US
dc.date.issued	2003-01-01	en_US
dc.identifier.citation	International Journal for Parasitology, 2003, 33 (12), pp. 1329 - 1340	en_US
dc.identifier.issn	0020-7519	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3904
dc.description.abstract	We have identified, and followed the development of three macrogamete organelles involved in the formation of the oocyst wall of Eimeria maxima. The first were small lucent vacuoles that cross-reacted with antibodies to the apple domains of the Toxoplasma gondii microneme protein 4. They appeared early in development and were secreted during macrogamete maturation to form an outer veil and were termed veil forming bodies. The second were the wall forming bodies type 1, large, electron dense vacuoles that stained positively only with antibodies raised to an enriched preparation of the native forms of 56 (gam56), 82 (gam82) and 230 kDa (gam230) gametocyte antigens (termed anti-APGA). The third were the wall forming bodies type 2, which appeared before the wall forming bodies type 1 but remain enclosed within the rough endoplasmic reticulum and stained positively with antibodies raised to recombinant versions of gam56 (anti-gam56), gam82 (anti-gam82) and gam230 (anti-gam230) plus anti-APGA. At the initiation of oocyst wall formation, the anti-T. gondii microneme protein 4 positive outer veil detached from the surface. The outer layer of the oocyst wall was formed by the release of the contents of wall forming bodies type 1 at the surface to form an electron dense, anti-APGA positive layer. The wall forming bodies type 2 appeared, subsequently, to give rise to the electron lucent inner layer. Thus, oocyst wall formation in E. maxima represents a sequential release of the contents of the veil forming bodies, wall forming bodies types 1 and 2 and this may be controlled at the level of the rough endoplasmic reticulum/Golgi body. © 2003 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.	en_US
dc.relation	http://purl.org/au-research/grants/arc/C19804392
dc.relation.ispartof	International Journal for Parasitology	en_US
dc.relation.isbasedon	10.1016/S0020-7519(03)00185-1	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Cell Wall	en_US
dc.subject.mesh	Organelles	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Chickens	en_US
dc.subject.mesh	Eimeria	en_US
dc.subject.mesh	Oocysts	en_US
dc.subject.mesh	Coccidiosis	en_US
dc.subject.mesh	Microscopy, Immunoelectron	en_US
dc.subject.mesh	Immunohistochemistry	en_US
dc.title	The development of the macrogamete and oocyst wall in Eimeria maxima: Immuno-light and electron microscopy	en_US
dc.type	Journal Article
utslib.citation.volume	12	en_US
utslib.citation.volume	33	en_US
utslib.for	0707 Veterinary Sciences	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	12	en_US
pubs.publication-status	Published	en_US
pubs.volume	33	en_US

Abstract:

We have identified, and followed the development of three macrogamete organelles involved in the formation of the oocyst wall of Eimeria maxima. The first were small lucent vacuoles that cross-reacted with antibodies to the apple domains of the Toxoplasma gondii microneme protein 4. They appeared early in development and were secreted during macrogamete maturation to form an outer veil and were termed veil forming bodies. The second were the wall forming bodies type 1, large, electron dense vacuoles that stained positively only with antibodies raised to an enriched preparation of the native forms of 56 (gam56), 82 (gam82) and 230 kDa (gam230) gametocyte antigens (termed anti-APGA). The third were the wall forming bodies type 2, which appeared before the wall forming bodies type 1 but remain enclosed within the rough endoplasmic reticulum and stained positively with antibodies raised to recombinant versions of gam56 (anti-gam56), gam82 (anti-gam82) and gam230 (anti-gam230) plus anti-APGA. At the initiation of oocyst wall formation, the anti-T. gondii microneme protein 4 positive outer veil detached from the surface. The outer layer of the oocyst wall was formed by the release of the contents of wall forming bodies type 1 at the surface to form an electron dense, anti-APGA positive layer. The wall forming bodies type 2 appeared, subsequently, to give rise to the electron lucent inner layer. Thus, oocyst wall formation in E. maxima represents a sequential release of the contents of the veil forming bodies, wall forming bodies types 1 and 2 and this may be controlled at the level of the rough endoplasmic reticulum/Golgi body. © 2003 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

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http://hdl.handle.net/10453/3904