Isolation of Neospora caninum genes detected during a chronic murine infection

Atkinson, RA; Ryce, C; Miller, CMD; Balu, S; Harper, PAW; Ellis, JT

Isolation of Neospora caninum genes detected during a chronic murine infection

Atkinson, RA Ryce, C Miller, CMD

Balu, S Harper, PAW Ellis, JT

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Publication Type:: Journal Article
Citation:: International Journal for Parasitology, 2001, 31 (1), pp. 67 - 71
Issue Date:: 2001-01-01

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Field	Value	Language
dc.contributor.author	Atkinson, RA	en_US
dc.contributor.author	Ryce, C	en_US
dc.contributor.author	Miller, CMD https://orcid.org/0000-0001-6071-5102	en_US
dc.contributor.author	Balu, S	en_US
dc.contributor.author	Harper, PAW	en_US
dc.contributor.author	Ellis, JT https://orcid.org/0000-0001-7328-4831	en_US
dc.date.issued	2001-01-01	en_US
dc.identifier.citation	International Journal for Parasitology, 2001, 31 (1), pp. 67 - 71	en_US
dc.identifier.issn	0020-7519	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3920
dc.description.abstract	In order to isolate genes coding for antigens of Neospora caninum which are recognised by the host immune system during a chronic murine infection, a cDNA library was immunoscreened with pooled sera from mice which survived three independent infections by N. caninum. Two new genes from N. caninum were isolated and expressed in Escherichia coli. The genes identified include one homologous to GRA1 of Toxoplasma gondii, plus another (NCP20) previously unknown in any taxon. Both genes encode small polypeptides which induced an IgG response in the mouse and were also recognised by IgG from a cow chronically infected with N. caninum. These results are consistent with the hypothesis that the polypeptides encoded by these genes are a target for the host immune system during chronic infections of N. caninum. © 2001 Published by Elsevier Science Ltd.	en_US
dc.relation.ispartof	International Journal for Parasitology	en_US
dc.relation.isbasedon	10.1016/S0020-7519(00)00153-3	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred BALB C	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Neospora	en_US
dc.subject.mesh	Toxoplasma	en_US
dc.subject.mesh	Coccidiosis	en_US
dc.subject.mesh	Rodent Diseases	en_US
dc.subject.mesh	DNA, Protozoan	en_US
dc.subject.mesh	DNA, Complementary	en_US
dc.subject.mesh	Antigens, Protozoan	en_US
dc.subject.mesh	Electrophoresis, Polyacrylamide Gel	en_US
dc.subject.mesh	Sequence Alignment	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Sequence Homology, Amino Acid	en_US
dc.subject.mesh	Open Reading Frames	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Isolation of Neospora caninum genes detected during a chronic murine infection	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	31	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	31	en_US

Abstract:

In order to isolate genes coding for antigens of Neospora caninum which are recognised by the host immune system during a chronic murine infection, a cDNA library was immunoscreened with pooled sera from mice which survived three independent infections by N. caninum. Two new genes from N. caninum were isolated and expressed in Escherichia coli. The genes identified include one homologous to GRA1 of Toxoplasma gondii, plus another (NCP20) previously unknown in any taxon. Both genes encode small polypeptides which induced an IgG response in the mouse and were also recognised by IgG from a cow chronically infected with N. caninum. These results are consistent with the hypothesis that the polypeptides encoded by these genes are a target for the host immune system during chronic infections of N. caninum. © 2001 Published by Elsevier Science Ltd.

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http://hdl.handle.net/10453/3920