Prevalence of neospora caninum infection in Australian (NSW) dairy cattle estimated by a newly validated ELISA for milk

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Journal Article
Veterinary Parasitology, 2006, 142 (1-2), pp. 173 - 178
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Aim: To determine the performance characteristics of an Institut Pourquier (IP) enzyme-linked immunosorbent assay (ELISA) for the detection of antobodies against Neospora caninum in bovine milk and subsequent determination of the prevalence of N. caninum infection in NSW dairy cattle. Conclusions: The prevalence of N.caninum in NSW dairy cattle was higher than previously believed. When used on individual milk samples this ELISA demonstrated high sensitivity and specificty and so could be used to accuratley identify N. caninum infection. TG-ROC analysis of the IP ELISA optimised the protocol and prescribed cut-off values enabling the ELISA to be used for the screening of N. caninum antibodies in the milk of dairy cattle.
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