Genus-optimized strategy for the identification of chlamydial type III secretion substrates

Hovis, KM; Mojica, S; Mcdermott, JE; Pedersen, L; Simhi, C; Rank, RG; Myers, GSA; Ravel, J; Hsia, RC; Bavoil, PM

Genus-optimized strategy for the identification of chlamydial type III secretion substrates

Hovis, KM Mojica, S Mcdermott, JE Pedersen, L Simhi, C Rank, RG Myers, GSA

Ravel, J Hsia, RC Bavoil, PM

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Publication Type:: Journal Article
Citation:: Pathogens and Disease, 2013, 69 (3), pp. 213 - 222
Issue Date:: 2013-01-01

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Field	Value	Language
dc.contributor.author	Hovis, KM	en_US
dc.contributor.author	Mojica, S	en_US
dc.contributor.author	Mcdermott, JE	en_US
dc.contributor.author	Pedersen, L	en_US
dc.contributor.author	Simhi, C	en_US
dc.contributor.author	Rank, RG	en_US
dc.contributor.author	Myers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Ravel, J	en_US
dc.contributor.author	Hsia, RC	en_US
dc.contributor.author	Bavoil, PM	en_US
dc.date.available	2013-07-09	en_US
dc.date.issued	2013-01-01	en_US
dc.identifier.citation	Pathogens and Disease, 2013, 69 (3), pp. 213 - 222	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39730
dc.description.abstract	Among chlamydial virulence factors are the type III secretion (T3S) system and its effectors. T3S effectors target host proteins to benefit the infecting chlamydiae. The assortment of effectors, each with a unique function, varies between species. This variation likely contributes to differences in host specificity and disease severity. A dozen effectors of Chlamydia trachomatis have been identified; however, estimates suggest that more exist. A T3S prediction algorithm, SVM-based Identification and Evaluation of Virulence Effectors (SIEVE), along with a Yersinia surrogate secretion system helped to identify a new T3S substrate, CT082, which rather than functioning as an effector associates with the chlamydial envelope after secretion. SIEVE was modified to improve/expand effector predictions to include all sequenced genomes. Additional adjustments were made to the existing surrogate system whereby the N terminus of putative effectors was fused to a known effector lacking its own N terminus and was tested for secretion. Expansion of effector predictions by cSIEVE and modification of the surrogate system have also assisted in identifying a new T3S substrate from C. psittaci. The expanded predictions along with modifications to improve the surrogate secretion system have enhanced our ability to identify novel species-specific effectors, which upon characterization should provide insight into the unique pathogenic properties of each species. © 2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.	en_US
dc.relation.ispartof	Pathogens and Disease	en_US
dc.relation.isbasedon	10.1111/2049-632X.12070	en_US
dc.subject.mesh	Cell Membrane	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Guinea Pigs	en_US
dc.subject.mesh	Chlamydia trachomatis	en_US
dc.subject.mesh	Yersinia pseudotuberculosis	en_US
dc.subject.mesh	Proteomics	en_US
dc.subject.mesh	Species Specificity	en_US
dc.subject.mesh	Gene Expression	en_US
dc.subject.mesh	Substrate Specificity	en_US
dc.subject.mesh	Protein Transport	en_US
dc.subject.mesh	Gene Order	en_US
dc.subject.mesh	Mutation	en_US
dc.subject.mesh	Bacterial Secretion Systems	en_US
dc.title	Genus-optimized strategy for the identification of chlamydial type III secretion substrates	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	69	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	69	en_US

Abstract:

Among chlamydial virulence factors are the type III secretion (T3S) system and its effectors. T3S effectors target host proteins to benefit the infecting chlamydiae. The assortment of effectors, each with a unique function, varies between species. This variation likely contributes to differences in host specificity and disease severity. A dozen effectors of Chlamydia trachomatis have been identified; however, estimates suggest that more exist. A T3S prediction algorithm, SVM-based Identification and Evaluation of Virulence Effectors (SIEVE), along with a Yersinia surrogate secretion system helped to identify a new T3S substrate, CT082, which rather than functioning as an effector associates with the chlamydial envelope after secretion. SIEVE was modified to improve/expand effector predictions to include all sequenced genomes. Additional adjustments were made to the existing surrogate system whereby the N terminus of putative effectors was fused to a known effector lacking its own N terminus and was tested for secretion. Expansion of effector predictions by cSIEVE and modification of the surrogate system have also assisted in identifying a new T3S substrate from C. psittaci. The expanded predictions along with modifications to improve the surrogate secretion system have enhanced our ability to identify novel species-specific effectors, which upon characterization should provide insight into the unique pathogenic properties of each species. © 2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

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http://hdl.handle.net/10453/39730