Comparison of koala LPCoLN and human strains of Chlamydia pneumoniae highlights extended genetic diversity in the species

Mitchell, CM; Hovis, KM; Bavoil, PM; Myers, GSA; Carrasco, JA; Timms, P

Comparison of koala LPCoLN and human strains of Chlamydia pneumoniae highlights extended genetic diversity in the species

Mitchell, CM Hovis, KM Bavoil, PM Myers, GSA

Carrasco, JA Timms, P

Permalink

Publication Type:: Journal Article
Citation:: BMC Genomics, 2010, 11 (1)
Issue Date:: 2010-07-21

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published VersionAdobe PDF (661.04 kB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Mitchell, CM	en_US
dc.contributor.author	Hovis, KM	en_US
dc.contributor.author	Bavoil, PM	en_US
dc.contributor.author	Myers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Carrasco, JA	en_US
dc.contributor.author	Timms, P	en_US
dc.date.available	2010-07-21	en_US
dc.date.issued	2010-07-21	en_US
dc.identifier.citation	BMC Genomics, 2010, 11 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39740
dc.description.abstract	Background: Chlamydia pneumoniae is a widespread pathogen causing upper and lower respiratory tract infections in addition to a range of other diseases in humans and animals. Previous whole genome analyses have focused on four essentially clonal (> 99% identity) C. pneumoniae human genomes (AR39, CWL029, J138 and TW183), providing relatively little insight into strain diversity and evolution of this species.Results: We performed individual gene-by-gene comparisons of the recently sequenced C. pneumoniae koala genome and four C. pneumoniae human genomes to identify species-specific genes, and more importantly, to gain an insight into the genetic diversity and evolution of the species. We selected genes dispersed throughout the chromosome, representing genes that were specific to C. pneumoniae, genes with a demonstrated role in chlamydial biology and/or pathogenicity (n = 49), genes encoding nucleotide salvage or amino acid biosynthesis proteins (n = 6), and extrachromosomal elements (9 plasmid and 2 bacteriophage genes).Conclusions: We have identified strain-specific differences and targets for detection of C. pneumoniae isolates from both human and animal origin. Such characterisation is necessary for an improved understanding of disease transmission and intervention. © 2010 Mitchell et al; licensee BioMed Central Ltd.	en_US
dc.relation.ispartof	BMC Genomics	en_US
dc.relation.isbasedon	10.1186/1471-2164-11-442	en_US
dc.subject.classification	Bioinformatics	en_US
dc.subject.mesh	Chromosomes, Bacterial	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Phascolarctidae	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Chlamydophila pneumoniae	en_US
dc.subject.mesh	Chlamydophila Infections	en_US
dc.subject.mesh	Nucleotides	en_US
dc.subject.mesh	Amino Acids	en_US
dc.subject.mesh	Adaptation, Physiological	en_US
dc.subject.mesh	Species Specificity	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Genetic Variation	en_US
dc.title	Comparison of koala LPCoLN and human strains of Chlamydia pneumoniae highlights extended genetic diversity in the species	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	11	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	08 Information and Computing Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	11	en_US

Abstract:

Background: Chlamydia pneumoniae is a widespread pathogen causing upper and lower respiratory tract infections in addition to a range of other diseases in humans and animals. Previous whole genome analyses have focused on four essentially clonal (> 99% identity) C. pneumoniae human genomes (AR39, CWL029, J138 and TW183), providing relatively little insight into strain diversity and evolution of this species.Results: We performed individual gene-by-gene comparisons of the recently sequenced C. pneumoniae koala genome and four C. pneumoniae human genomes to identify species-specific genes, and more importantly, to gain an insight into the genetic diversity and evolution of the species. We selected genes dispersed throughout the chromosome, representing genes that were specific to C. pneumoniae, genes with a demonstrated role in chlamydial biology and/or pathogenicity (n = 49), genes encoding nucleotide salvage or amino acid biosynthesis proteins (n = 6), and extrachromosomal elements (9 plasmid and 2 bacteriophage genes).Conclusions: We have identified strain-specific differences and targets for detection of C. pneumoniae isolates from both human and animal origin. Such characterisation is necessary for an improved understanding of disease transmission and intervention. © 2010 Mitchell et al; licensee BioMed Central Ltd.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/39740