Genomic analysis of an attenuated Chlamydia abortus live vaccine strain reveals defects in central metabolism and surface proteins

Burall, LS; Rodolakis, A; Rekiki, A; Myers, GSA; Bavoil, PM

Genomic analysis of an attenuated Chlamydia abortus live vaccine strain reveals defects in central metabolism and surface proteins

Burall, LS Rodolakis, A Rekiki, A Myers, GSA

Bavoil, PM

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Publication Type:: Journal Article
Citation:: Infection and Immunity, 2009, 77 (9), pp. 4161 - 4167
Issue Date:: 2009-09-01

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Field	Value	Language
dc.contributor.author	Burall, LS	en_US
dc.contributor.author	Rodolakis, A	en_US
dc.contributor.author	Rekiki, A	en_US
dc.contributor.author	Myers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Bavoil, PM	en_US
dc.date.issued	2009-09-01	en_US
dc.identifier.citation	Infection and Immunity, 2009, 77 (9), pp. 4161 - 4167	en_US
dc.identifier.issn	0019-9567	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39743
dc.description.abstract	Comparative genomic analysis of a wild-type strain of the ovine pathogen Chlamydia abortus and its nitrosoguanidine-induced, temperature-sensitive, virulence-attenuated live vaccine derivative identified 22 single nucleotide polymorphisms unique to the mutant, including nine nonsynonymous mutations, one leading to a truncation of pmpG, which encodes a polymorphic membrane protein, and two intergenic mutations potentially affecting promoter sequences. Other nonsynonymous mutations mapped to a pmpG pseudogene and to predicted coding sequences encoding a putative lipoprotein, a sigma-54-dependent response regulator, a PhoH-like protein, a putative export protein, two tRNA synthetases, and a putative serine hydroxymethyltransferase. One of the intergenic mutations putatively affects transcription of two divergent genes encoding pyruvate kinase and a putative SOS response nuclease, respectively. These observations suggest that the temperature-sensitive phenotype and associated virulence attenuation of the vaccine strain result from disrupted metabolic activity due to altered pyruvate kinase expression and/or alteration in the function of one or more membrane proteins, most notably PmpG and a putative lipoprotein. Copyright © 2009, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Infection and Immunity	en_US
dc.relation.isbasedon	10.1128/IAI.00189-09	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Chlamydophila psittaci	en_US
dc.subject.mesh	Cephalosporins	en_US
dc.subject.mesh	Methionine-tRNA Ligase	en_US
dc.subject.mesh	Glycine Hydroxymethyltransferase	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Membrane Proteins	en_US
dc.subject.mesh	Bacterial Vaccines	en_US
dc.subject.mesh	Vaccines, Attenuated	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Mutation	en_US
dc.subject.mesh	Polymorphism, Single Nucleotide	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.title	Genomic analysis of an attenuated Chlamydia abortus live vaccine strain reveals defects in central metabolism and surface proteins	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	77	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	77	en_US

Abstract:

Comparative genomic analysis of a wild-type strain of the ovine pathogen Chlamydia abortus and its nitrosoguanidine-induced, temperature-sensitive, virulence-attenuated live vaccine derivative identified 22 single nucleotide polymorphisms unique to the mutant, including nine nonsynonymous mutations, one leading to a truncation of pmpG, which encodes a polymorphic membrane protein, and two intergenic mutations potentially affecting promoter sequences. Other nonsynonymous mutations mapped to a pmpG pseudogene and to predicted coding sequences encoding a putative lipoprotein, a sigma-54-dependent response regulator, a PhoH-like protein, a putative export protein, two tRNA synthetases, and a putative serine hydroxymethyltransferase. One of the intergenic mutations putatively affects transcription of two divergent genes encoding pyruvate kinase and a putative SOS response nuclease, respectively. These observations suggest that the temperature-sensitive phenotype and associated virulence attenuation of the vaccine strain result from disrupted metabolic activity due to altered pyruvate kinase expression and/or alteration in the function of one or more membrane proteins, most notably PmpG and a putative lipoprotein. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/39743