Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens

Myers, GSA; Rasko, DA; Cheung, JK; Ravel, J; Seshadri, R; DeBoy, RT; Ren, Q; Varga, J; Awad, MM; Brinkac, LM; Daugherty, SC; Haft, DH; Dodson, RJ; Madupu, R; Nelson, WC; Rosovitz, MJ; Sullivan, SA; Khouri, H; Dimitrov, GI; Watkins, KL; Mulligan, S; Benton, J; Radune, D; Fisher, DJ; Atkins, HS; Hiscox, T; Jost, BH; Billington, SJ; Songer, JG; McClane, BA; Titball, RW; Rood, JI; Melville, SB; Paulsen, IT

Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens

Rasko, DA Cheung, JK Ravel, J Seshadri, R DeBoy, RT Ren, Q Varga, J Awad, MM Brinkac, LM Daugherty, SC Haft, DH Dodson, RJ Madupu, R Nelson, WC Rosovitz, MJ Sullivan, SA Khouri, H Dimitrov, GI Watkins, KL Mulligan, S Benton, J Radune, D Fisher, DJ Atkins, HS Hiscox, T Jost, BH Billington, SJ Songer, JG McClane, BA Titball, RW Rood, JI Melville, SB Paulsen, IT

Permalink

Publication Type:: Journal Article
Citation:: Genome Research, 2006, 16 (8), pp. 1031 - 1040
Issue Date:: 2006-08-08

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published VersionAdobe PDF (799.03 kB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Myers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Rasko, DA	en_US
dc.contributor.author	Cheung, JK	en_US
dc.contributor.author	Ravel, J	en_US
dc.contributor.author	Seshadri, R	en_US
dc.contributor.author	DeBoy, RT	en_US
dc.contributor.author	Ren, Q	en_US
dc.contributor.author	Varga, J	en_US
dc.contributor.author	Awad, MM	en_US
dc.contributor.author	Brinkac, LM	en_US
dc.contributor.author	Daugherty, SC	en_US
dc.contributor.author	Haft, DH	en_US
dc.contributor.author	Dodson, RJ	en_US
dc.contributor.author	Madupu, R	en_US
dc.contributor.author	Nelson, WC	en_US
dc.contributor.author	Rosovitz, MJ	en_US
dc.contributor.author	Sullivan, SA	en_US
dc.contributor.author	Khouri, H	en_US
dc.contributor.author	Dimitrov, GI	en_US
dc.contributor.author	Watkins, KL	en_US
dc.contributor.author	Mulligan, S	en_US
dc.contributor.author	Benton, J	en_US
dc.contributor.author	Radune, D	en_US
dc.contributor.author	Fisher, DJ	en_US
dc.contributor.author	Atkins, HS	en_US
dc.contributor.author	Hiscox, T	en_US
dc.contributor.author	Jost, BH	en_US
dc.contributor.author	Billington, SJ	en_US
dc.contributor.author	Songer, JG	en_US
dc.contributor.author	McClane, BA	en_US
dc.contributor.author	Titball, RW	en_US
dc.contributor.author	Rood, JI	en_US
dc.contributor.author	Melville, SB	en_US
dc.contributor.author	Paulsen, IT	en_US
dc.date.issued	2006-08-08	en_US
dc.identifier.citation	Genome Research, 2006, 16 (8), pp. 1031 - 1040	en_US
dc.identifier.issn	1088-9051	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39758
dc.description.abstract	Clostridium perfringens is a Gram-positive, anaerobic spore-forming bacterium commonly found in soil, sediments, and the human gastrointestinal tract. C. perfringens is responsible for a wide spectrum of disease, including food poisoning, gas gangrene (clostridial myonecrosis), enteritis necroticans, and non-foodborne gastrointestinal infections. The complete genome sequences of Clostridium perfringens strain ATCC 13124, a gas gangrene isolate and the species type strain, and the enterotoxin-producing food poisoning strain SM101, were determined and compared with the published C. perfringens strain 13 genome. Comparison of the three genomes revealed considerable genomic diversity with >300 unique "genomic islands" identified, with the majority of these islands unusually clustered on one replichore. PCR-based analysis indicated that the large genomic islands are widely variable across a large collection of C. perfringens strains. These islands encode genes that correlate to differences in virulence and phenotypic characteristics of these strains. Significant differences between the strains include numerous novel mobile elements and genes encoding metabolic capabilities, strain-specific extracellular polysaccharide capsule, sporulation factors, toxins, and other secreted enzymes, providing substantial insight into this medically important bacterial pathogen. ©2006 by Cold Spring Harbor Laboratory Press.	en_US
dc.relation.ispartof	Genome Research	en_US
dc.relation.isbasedon	10.1101/gr.5238106	en_US
dc.subject.classification	Bioinformatics	en_US
dc.subject.mesh	Clostridium perfringens	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Bacterial Toxins	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	16	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	16	en_US

Abstract:

Clostridium perfringens is a Gram-positive, anaerobic spore-forming bacterium commonly found in soil, sediments, and the human gastrointestinal tract. C. perfringens is responsible for a wide spectrum of disease, including food poisoning, gas gangrene (clostridial myonecrosis), enteritis necroticans, and non-foodborne gastrointestinal infections. The complete genome sequences of Clostridium perfringens strain ATCC 13124, a gas gangrene isolate and the species type strain, and the enterotoxin-producing food poisoning strain SM101, were determined and compared with the published C. perfringens strain 13 genome. Comparison of the three genomes revealed considerable genomic diversity with >300 unique "genomic islands" identified, with the majority of these islands unusually clustered on one replichore. PCR-based analysis indicated that the large genomic islands are widely variable across a large collection of C. perfringens strains. These islands encode genes that correlate to differences in virulence and phenotypic characteristics of these strains. Significant differences between the strains include numerous novel mobile elements and genes encoding metabolic capabilities, strain-specific extracellular polysaccharide capsule, sporulation factors, toxins, and other secreted enzymes, providing substantial insight into this medically important bacterial pathogen. ©2006 by Cold Spring Harbor Laboratory Press.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/39758