Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis

Paulsen, IT; Banerjei, L; Hyers, GSA; Nelson, KE; Seshadri, R; Read, TD; Fouts, DE; Eisen, JA; Gill, SR; Heidelberg, JF; Tettelin, H; Dodson, RJ; Umayam, L; Brinkac, L; Beanan, M; Daugherty, S; DeBoy, RT; Durkin, S; Kolonay, J; Madupu, R; Nelson, W; Vamathevan, J; Tran, B; Upton, J; Hansen, T; Shetty, J; Khouri, H; Utterback, T; Radune, D; Ketchum, KA; Dougherty, BA; Fraser, CM

Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis

Paulsen, IT Banerjei, L Hyers, GSA

Nelson, KE Seshadri, R Read, TD Fouts, DE Eisen, JA Gill, SR Heidelberg, JF Tettelin, H Dodson, RJ Umayam, L Brinkac, L Beanan, M Daugherty, S DeBoy, RT Durkin, S Kolonay, J Madupu, R Nelson, W Vamathevan, J Tran, B Upton, J Hansen, T Shetty, J Khouri, H Utterback, T Radune, D Ketchum, KA Dougherty, BA Fraser, CM

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Publication Type:: Journal Article
Citation:: Science, 2003, 299 (5615), pp. 2071 - 2074
Issue Date:: 2003-03-28

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Field	Value	Language
dc.contributor.author	Paulsen, IT	en_US
dc.contributor.author	Banerjei, L	en_US
dc.contributor.author	Hyers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Nelson, KE	en_US
dc.contributor.author	Seshadri, R	en_US
dc.contributor.author	Read, TD	en_US
dc.contributor.author	Fouts, DE	en_US
dc.contributor.author	Eisen, JA	en_US
dc.contributor.author	Gill, SR	en_US
dc.contributor.author	Heidelberg, JF	en_US
dc.contributor.author	Tettelin, H	en_US
dc.contributor.author	Dodson, RJ	en_US
dc.contributor.author	Umayam, L	en_US
dc.contributor.author	Brinkac, L	en_US
dc.contributor.author	Beanan, M	en_US
dc.contributor.author	Daugherty, S	en_US
dc.contributor.author	DeBoy, RT	en_US
dc.contributor.author	Durkin, S	en_US
dc.contributor.author	Kolonay, J	en_US
dc.contributor.author	Madupu, R	en_US
dc.contributor.author	Nelson, W	en_US
dc.contributor.author	Vamathevan, J	en_US
dc.contributor.author	Tran, B	en_US
dc.contributor.author	Upton, J	en_US
dc.contributor.author	Hansen, T	en_US
dc.contributor.author	Shetty, J	en_US
dc.contributor.author	Khouri, H	en_US
dc.contributor.author	Utterback, T	en_US
dc.contributor.author	Radune, D	en_US
dc.contributor.author	Ketchum, KA	en_US
dc.contributor.author	Dougherty, BA	en_US
dc.contributor.author	Fraser, CM	en_US
dc.date.issued	2003-03-28	en_US
dc.identifier.citation	Science, 2003, 299 (5615), pp. 2071 - 2074	en_US
dc.identifier.issn	0036-8075	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39769
dc.description.abstract	The complete genome sequence of Enterococcus faecalis V583, a vancomycin-resistant clinical isolate, revealed that more than a quarter of the genome consists of probable mobile or foreign DNA. One of the predicted mobile elements is a previously unknown vanB vancomycin-resistance conjugative transposon. Three plasmids were identified, including two pheromone-sensing conjugative plasmids, one encoding a previously undescribed pheromone inhibitor. The apparent propensity for the incorporation of mobile elements probably contributed to the rapid acquisition and dissemination of drug resistance in the enterococci.	en_US
dc.relation.ispartof	Science	en_US
dc.relation.isbasedon	10.1126/science.1080613	en_US
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Digestive System	en_US
dc.subject.mesh	Chromosomes, Bacterial	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Enterococcus faecalis	en_US
dc.subject.mesh	Gram-Positive Bacterial Infections	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Adhesins, Bacterial	en_US
dc.subject.mesh	Carrier Proteins	en_US
dc.subject.mesh	DNA Transposable Elements	en_US
dc.subject.mesh	Virulence Factors	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Bacterial Adhesion	en_US
dc.subject.mesh	Drug Resistance, Multiple, Bacterial	en_US
dc.subject.mesh	Vancomycin Resistance	en_US
dc.subject.mesh	Lysogeny	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Conjugation, Genetic	en_US
dc.subject.mesh	Gene Transfer, Horizontal	en_US
dc.subject.mesh	Interspersed Repetitive Sequences	en_US
dc.subject.mesh	Conserved Sequence	en_US
dc.subject.mesh	Synteny	en_US
dc.subject.mesh	Oxidative Stress	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.subject.mesh	Open Reading Frames	en_US
dc.subject.mesh	Plasmids	en_US
dc.subject.mesh	Biological Evolution	en_US
dc.title	Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis	en_US
dc.type	Journal Article
utslib.citation.volume	5615	en_US
utslib.citation.volume	299	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	5615	en_US
pubs.publication-status	Published	en_US
pubs.volume	299	en_US

Abstract:

The complete genome sequence of Enterococcus faecalis V583, a vancomycin-resistant clinical isolate, revealed that more than a quarter of the genome consists of probable mobile or foreign DNA. One of the predicted mobile elements is a previously unknown vanB vancomycin-resistance conjugative transposon. Three plasmids were identified, including two pheromone-sensing conjugative plasmids, one encoding a previously undescribed pheromone inhibitor. The apparent propensity for the incorporation of mobile elements probably contributed to the rapid acquisition and dissemination of drug resistance in the enterococci.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/39769