Expression of two novel proteins in Chlamydia trachomatis during natural infection

Myers, GSA; Grinvalds, R; Booth, S; Hutton, SI; Binks, M; Kemp, DJ; Sriprakash, KS

Expression of two novel proteins in Chlamydia trachomatis during natural infection

Myers, GSA

Grinvalds, R Booth, S Hutton, SI Binks, M Kemp, DJ Sriprakash, KS

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Publication Type:: Journal Article
Citation:: Microbial Pathogenesis, 2000, 29 (2), pp. 63 - 72
Issue Date:: 2000-01-01

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Field	Value	Language
dc.contributor.author	Myers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Grinvalds, R	en_US
dc.contributor.author	Booth, S	en_US
dc.contributor.author	Hutton, SI	en_US
dc.contributor.author	Binks, M	en_US
dc.contributor.author	Kemp, DJ	en_US
dc.contributor.author	Sriprakash, KS	en_US
dc.date.issued	2000-01-01	en_US
dc.identifier.citation	Microbial Pathogenesis, 2000, 29 (2), pp. 63 - 72	en_US
dc.identifier.issn	0882-4010	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39770
dc.description.abstract	Genes for a putative membrane associated protein (mvi-homologue) and a 48 kDa protein (ctr48) in Chlamydia trachomatis were characterized. The mvi-homologue has 12 transmembrane domains and shows considerable homology to the members of this gene family in various organisms. The ctr48 has a leader sequence and the C-proximal half is tryptophan-rich. The latter region shares 65% identity with the N-proxima third of C. pneumoniae 76 kDa protein over an overlap of 231 amino acid residues. The genes for the mvi-homologue and the ctr48 are present in the B, Ba, D, E, J and L2 serotypes of C. trachomatis. Immediately downstream from the ctr48 gene are multiple stop codons which are followed by a functional rho-independent terminator. The mvi-homologue and ctr48 genes are independently transcribed, albeit poorly in serotype B. However, protein products corresponding to these genes could not be detected by western blotting in HEp2 cells infected with C. trachomatis. Nevertheless, antibodies to peptides corresponding to these proteins were detected in sera with high micro-immunofluorescence titre against C. trachomatic, collected from a Chlamydia-endemic population. These results suggest that the mvi-homologue and ctr48 are expressed by C. trachomatis during natural infection. (C) 2000 Academic Press.	en_US
dc.relation.ispartof	Microbial Pathogenesis	en_US
dc.relation.isbasedon	10.1006/mpat.2000.0359	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Chlamydia trachomatis	en_US
dc.subject.mesh	Chlamydia Infections	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Membrane Proteins	en_US
dc.subject.mesh	RNA, Bacterial	en_US
dc.subject.mesh	Antibodies, Bacterial	en_US
dc.subject.mesh	Immunoblotting	en_US
dc.subject.mesh	Reverse Transcriptase Polymerase Chain Reaction	en_US
dc.subject.mesh	Transcription, Genetic	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Infant	en_US
dc.subject.mesh	Female	en_US
dc.title	Expression of two novel proteins in Chlamydia trachomatis during natural infection	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	29	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1107 Immunology	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	29	en_US

Abstract:

Genes for a putative membrane associated protein (mvi-homologue) and a 48 kDa protein (ctr48) in Chlamydia trachomatis were characterized. The mvi-homologue has 12 transmembrane domains and shows considerable homology to the members of this gene family in various organisms. The ctr48 has a leader sequence and the C-proximal half is tryptophan-rich. The latter region shares 65% identity with the N-proxima third of C. pneumoniae 76 kDa protein over an overlap of 231 amino acid residues. The genes for the mvi-homologue and the ctr48 are present in the B, Ba, D, E, J and L2 serotypes of C. trachomatis. Immediately downstream from the ctr48 gene are multiple stop codons which are followed by a functional rho-independent terminator. The mvi-homologue and ctr48 genes are independently transcribed, albeit poorly in serotype B. However, protein products corresponding to these genes could not be detected by western blotting in HEp2 cells infected with C. trachomatis. Nevertheless, antibodies to peptides corresponding to these proteins were detected in sera with high micro-immunofluorescence titre against C. trachomatic, collected from a Chlamydia-endemic population. These results suggest that the mvi-homologue and ctr48 are expressed by C. trachomatis during natural infection. (C) 2000 Academic Press.

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http://hdl.handle.net/10453/39770