A two-component regulatory system modulates twitching motility in Dichelobacter nodosus

Kennan, RM; Lovitt, CJ; Han, X; Parker, D; Turnbull, L; Whitchurch, CB; Rood, JI

A two-component regulatory system modulates twitching motility in Dichelobacter nodosus

Kennan, RM Lovitt, CJ Han, X Parker, D Turnbull, L

Whitchurch, CB

Rood, JI

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Publication Type:: Journal Article
Citation:: Veterinary Microbiology, 2015, 179 (1-2), pp. 34 - 41
Issue Date:: 2015-01-01

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Field	Value	Language
dc.contributor.author	Kennan, RM	en_US
dc.contributor.author	Lovitt, CJ	en_US
dc.contributor.author	Han, X	en_US
dc.contributor.author	Parker, D	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Rood, JI	en_US
dc.date.available	2020-05-25T19:17:23Z
dc.date.issued	2015-01-01	en_US
dc.identifier.citation	Veterinary Microbiology, 2015, 179 (1-2), pp. 34 - 41	en_US
dc.identifier.issn	0378-1135	en_US
dc.identifier.uri	http://hdl.handle.net/10453/41113
dc.description.abstract	© 2015 Elsevier B.V. All rights reserved. Dichelobacter nodosus is the essential causative agent of footrot in sheep and type IV fimbriae-mediated twitching motility has been shownto be essential for virulence.Wehave identified a two-component signal transduction system (TwmSR) that shows similarity to chemosensory systems from other bacteria. Insertional inactivation of the gene encoding the response regulator, TwmR, led to a twitching motility defect, with the mutant having a reduced rate of twitching motility when compared to the wild-type and a mutant complemented with the wild-type twmR gene. The reduced rate of twitching motility was not a consequence of a reduced growth rate or decreased production of surface located fimbriae, but video microscopy indicated that it appeared to result from an overall loss of twitching directionality. These results suggest that a chemotactic response to environmental factors may play an important role in the D. nodosus-mediated disease process.	en_US
dc.relation.ispartof	Veterinary Microbiology	en_US
dc.relation.isbasedon	10.1016/j.vetmic.2015.03.025	en_US
dc.subject.classification	Veterinary Sciences	en_US
dc.subject.mesh	Fimbriae, Bacterial	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Sheep	en_US
dc.subject.mesh	Dichelobacter nodosus	en_US
dc.subject.mesh	Gram-Negative Bacterial Infections	en_US
dc.subject.mesh	Foot Rot	en_US
dc.subject.mesh	Sheep Diseases	en_US
dc.subject.mesh	Mutagenesis, Insertional	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Signal Transduction	en_US
dc.subject.mesh	Chemotaxis	en_US
dc.title	A two-component regulatory system modulates twitching motility in Dichelobacter nodosus	en_US
dc.type	Journal Article
utslib.citation.volume	1-2	en_US
utslib.citation.volume	179	en_US
utslib.for	0707 Veterinary Sciences	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	1-2	en_US
pubs.publication-status	Published	en_US
pubs.volume	179	en_US

Abstract:

© 2015 Elsevier B.V. All rights reserved. Dichelobacter nodosus is the essential causative agent of footrot in sheep and type IV fimbriae-mediated twitching motility has been shownto be essential for virulence.Wehave identified a two-component signal transduction system (TwmSR) that shows similarity to chemosensory systems from other bacteria. Insertional inactivation of the gene encoding the response regulator, TwmR, led to a twitching motility defect, with the mutant having a reduced rate of twitching motility when compared to the wild-type and a mutant complemented with the wild-type twmR gene. The reduced rate of twitching motility was not a consequence of a reduced growth rate or decreased production of surface located fimbriae, but video microscopy indicated that it appeared to result from an overall loss of twitching directionality. These results suggest that a chemotactic response to environmental factors may play an important role in the D. nodosus-mediated disease process.

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http://hdl.handle.net/10453/41113