Multifunctional chitosan/polyvinyl pyrrolidone/45S5 Bioglass® scaffolds for MC3T3-E1 cell stimulation and drug release

Yao, Q; Li, W; Yu, S; Ma, L; Jin, D; Boccaccini, AR; Liu, Y

Multifunctional chitosan/polyvinyl pyrrolidone/45S5 Bioglass® scaffolds for MC3T3-E1 cell stimulation and drug release

Yao, Q Li, W Yu, S Ma, L Jin, D

Boccaccini, AR Liu, Y

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Publication Type:: Journal Article
Citation:: Materials Science and Engineering C, 2015, 56 pp. 473 - 480
Issue Date:: 2015-07-20

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Field	Value	Language
dc.contributor.author	Yao, Q	en_US
dc.contributor.author	Li, W	en_US
dc.contributor.author	Yu, S	en_US
dc.contributor.author	Ma, L	en_US
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666	en_US
dc.contributor.author	Boccaccini, AR	en_US
dc.contributor.author	Liu, Y	en_US
dc.date.available	2015-06-23	en_US
dc.date.issued	2015-07-20	en_US
dc.identifier.citation	Materials Science and Engineering C, 2015, 56 pp. 473 - 480	en_US
dc.identifier.issn	0928-4931	en_US
dc.identifier.uri	http://hdl.handle.net/10453/41180
dc.description.abstract	© 2015 Published by Elsevier B.V. Abstract Novel chitosan-polyvinyl pyrrolidone/45S5 Bioglass® (CS-PVP/BG) scaffolds were prepared via foam replication and chemical cross-linking techniques. The pristine BG, CS-PVP coated BG and genipin cross-linked CS-PVP/BG (G-CS-PVP/BG) scaffolds were synthesized and characterized in terms of chemical composition, physical structure and morphology respectively. Resistance to enzymatic degradation of the scaffold is improved significantly with the use of genipin cross-linked CS-PVP. The bio-effects of scaffolds on MC3T3-E1 osteoblast-like cells were evaluated by studying cell viability, adhesion and proliferation. The CCK-8 assay shows that cell viability on the resulting G-CS-PVP/BG scaffold is improved obviously after cross-linking of genipin. Cell skeleton images exhibit that well-stretched F-actin bundles are obtained on the G-CS-PVP/BG scaffold. SEM results present significant improvement on the cell adhesion and proliferation for cells cultured on the G-CS-PVP/BG scaffold. The drug release performance on the as-synthesized scaffold was studied in a phosphate buffered saline (PBS) solution. Vancomycin is found to be released in burst fashion within 24 h from the pristine BG scaffold, however, the release period from the G-CS-PVP/BG scaffold is enhanced to 7 days, indicating improved drug release properties of the G-CS-PVP/BG scaffold. Our results suggest that the G-CS-PVP/BG scaffolds possess promising physicochemical properties, sustained drug release capability and good biocompatibility for MC3T3-E1 cells' proliferation and adhesion, suggesting their potential applications in areas such as MC3T3-E1 cell stimulation and bone tissue engineering.	en_US
dc.relation.ispartof	Materials Science and Engineering C	en_US
dc.relation.isbasedon	10.1016/j.msec.2015.06.046	en_US
dc.subject.classification	Biomedical Engineering	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Osteoblasts	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Povidone	en_US
dc.subject.mesh	Iridoids	en_US
dc.subject.mesh	Chitosan	en_US
dc.subject.mesh	Glass	en_US
dc.subject.mesh	Ceramics	en_US
dc.subject.mesh	Delayed-Action Preparations	en_US
dc.subject.mesh	Cell Adhesion	en_US
dc.subject.mesh	Cell Proliferation	en_US
dc.title	Multifunctional chitosan/polyvinyl pyrrolidone/45S5 Bioglass® scaffolds for MC3T3-E1 cell stimulation and drug release	en_US
dc.type	Journal Article
utslib.citation.volume	56	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0912 Materials Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	56	en_US

Abstract:

© 2015 Published by Elsevier B.V. Abstract Novel chitosan-polyvinyl pyrrolidone/45S5 Bioglass® (CS-PVP/BG) scaffolds were prepared via foam replication and chemical cross-linking techniques. The pristine BG, CS-PVP coated BG and genipin cross-linked CS-PVP/BG (G-CS-PVP/BG) scaffolds were synthesized and characterized in terms of chemical composition, physical structure and morphology respectively. Resistance to enzymatic degradation of the scaffold is improved significantly with the use of genipin cross-linked CS-PVP. The bio-effects of scaffolds on MC3T3-E1 osteoblast-like cells were evaluated by studying cell viability, adhesion and proliferation. The CCK-8 assay shows that cell viability on the resulting G-CS-PVP/BG scaffold is improved obviously after cross-linking of genipin. Cell skeleton images exhibit that well-stretched F-actin bundles are obtained on the G-CS-PVP/BG scaffold. SEM results present significant improvement on the cell adhesion and proliferation for cells cultured on the G-CS-PVP/BG scaffold. The drug release performance on the as-synthesized scaffold was studied in a phosphate buffered saline (PBS) solution. Vancomycin is found to be released in burst fashion within 24 h from the pristine BG scaffold, however, the release period from the G-CS-PVP/BG scaffold is enhanced to 7 days, indicating improved drug release properties of the G-CS-PVP/BG scaffold. Our results suggest that the G-CS-PVP/BG scaffolds possess promising physicochemical properties, sustained drug release capability and good biocompatibility for MC3T3-E1 cells' proliferation and adhesion, suggesting their potential applications in areas such as MC3T3-E1 cell stimulation and bone tissue engineering.

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http://hdl.handle.net/10453/41180