Targeted destruction of murine macrophage cells with bioconjugated gold nanorods

Publication Type:
Journal Article
Citation:
Journal of Nanoparticle Research, 2007, 9 (6), pp. 1109 - 1124
Issue Date:
2007-12-01
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Gold nanorods manifest a readily tunable longitudinal plasmon resonance with light and consequently have potential for use in photothermal therapeutics. Recent work by others has shown how gold nanoshells and rods can be used to target cancer cells, which can then be destroyed using relatively high power laser radiation (∼1×105to 1×1010W/m2). Here we extend this concept to demonstrate how gold nanorods can be modified to bind to target macrophage cells, and show that high intensity laser radiation is not necessary, with even 5×102W/m2being sufficient, provided that a total fluence of ∼30 J/cm2is delivered. We used the murine cell line RAW 264.7 and the monoclonal antibody CD11b, raised against murine macrophages, as our model system and a 5 mW solid state diode laser as our energy source. Exposure of the cells labeled with gold nanorods to a laser fluence of 30 J/cm2resulted in 81% cell death compared to only 0.9% in the control, non-labeled cells. © 2007 Springer Science+Business Media, Inc.
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