Vibrio sp. as a potentially important member of the Black Band Disease (BBD) consortium in Favia sp. corals

Arotsker, L; Siboni, N; Ben-Dov, E; Kramarsky-Winter, E; Loya, Y; Kushmaro, A

Vibrio sp. as a potentially important member of the Black Band Disease (BBD) consortium in Favia sp. corals

Arotsker, L Siboni, N

Ben-Dov, E Kramarsky-Winter, E Loya, Y

Kushmaro, A

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Publication Type:: Journal Article
Citation:: FEMS Microbiology Ecology, 2009, 70 (3), pp. 515 - 524
Issue Date:: 2009-12-01

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Field	Value	Language
dc.contributor.author	Arotsker, L	en_US
dc.contributor.author	Siboni, N https://orcid.org/0000-0001-6082-0949	en_US
dc.contributor.author	Ben-Dov, E	en_US
dc.contributor.author	Kramarsky-Winter, E	en_US
dc.contributor.author	Loya, Y https://orcid.org/0000-0001-6870-9444	en_US
dc.contributor.author	Kushmaro, A https://orcid.org/0000-0002-1970-9656	en_US
dc.date.issued	2009-12-01	en_US
dc.identifier.citation	FEMS Microbiology Ecology, 2009, 70 (3), pp. 515 - 524	en_US
dc.identifier.issn	0168-6496	en_US
dc.identifier.uri	http://hdl.handle.net/10453/43885
dc.description.abstract	Black Band Disease (BBD) is a well-described disease plaguing corals worldwide. It has been established that ecological and environmental stress factors contribute to the appearance and progression of the disease, believed to be caused by a diverse microbial consortium. We have identified and characterized Vibrio sp. associated with BBD in Eilat reef corals using both culture-dependent and -independent methods. Direct sampling using 16S rRNA gene clone libraries showed seasonal dynamics in the diversity of BBD-associated Vibrios. In the two sampling periods, BBD-associated Vibrio clones showed similarities to different groups: October samples were similar to known pathogens, while December samples were similar to general aquatic Vibrio sp. Cultured bacterial isolates of Vibrio sp. were highly homologous (≥99%) to previously documented BBD-associated bacteria from the Caribbean, Bahamas and Red Seas, and were similar to several known coral pathogens, such as Vibrio coralliilyticus. The proteolytic activity of Vibrio sp., as measured using casein- and azocasein-based assays, directly correlated with temperature elevation and peaked at 26-28 °C, with the microorganisms producing more proteases per bacterial cell or increasing the rate of proteolytic activity of the same proteases (potentially metalloproteases). This activity may promote coral tissue necrosis and aid in ensuing progression of the coral BBD. © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.	en_US
dc.relation.ispartof	FEMS Microbiology Ecology	en_US
dc.relation.isbasedon	10.1111/j.1574-6941.2009.00770.x	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Anthozoa	en_US
dc.subject.mesh	Vibrio	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Biodiversity	en_US
dc.subject.mesh	Seasons	en_US
dc.subject.mesh	Seawater	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Gene Library	en_US
dc.subject.mesh	Indian Ocean	en_US
dc.title	Vibrio sp. as a potentially important member of the Black Band Disease (BBD) consortium in Favia sp. corals	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	70	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	70	en_US

Abstract:

Black Band Disease (BBD) is a well-described disease plaguing corals worldwide. It has been established that ecological and environmental stress factors contribute to the appearance and progression of the disease, believed to be caused by a diverse microbial consortium. We have identified and characterized Vibrio sp. associated with BBD in Eilat reef corals using both culture-dependent and -independent methods. Direct sampling using 16S rRNA gene clone libraries showed seasonal dynamics in the diversity of BBD-associated Vibrios. In the two sampling periods, BBD-associated Vibrio clones showed similarities to different groups: October samples were similar to known pathogens, while December samples were similar to general aquatic Vibrio sp. Cultured bacterial isolates of Vibrio sp. were highly homologous (≥99%) to previously documented BBD-associated bacteria from the Caribbean, Bahamas and Red Seas, and were similar to several known coral pathogens, such as Vibrio coralliilyticus. The proteolytic activity of Vibrio sp., as measured using casein- and azocasein-based assays, directly correlated with temperature elevation and peaked at 26-28 °C, with the microorganisms producing more proteases per bacterial cell or increasing the rate of proteolytic activity of the same proteases (potentially metalloproteases). This activity may promote coral tissue necrosis and aid in ensuing progression of the coral BBD. © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

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http://hdl.handle.net/10453/43885