Global distribution and diversity of coral-associated Archaea and their possible role in the coral holobiont nitrogen cycle

Siboni, N; Ben-Dov, E; Sivan, A; Kushmaro, A

Global distribution and diversity of coral-associated Archaea and their possible role in the coral holobiont nitrogen cycle

Siboni, N

Ben-Dov, E Sivan, A Kushmaro, A

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Publication Type:: Journal Article
Citation:: Environmental Microbiology, 2008, 10 (11), pp. 2979 - 2990
Issue Date:: 2008-11-01

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Field	Value	Language
dc.contributor.author	Siboni, N https://orcid.org/0000-0001-6082-0949	en_US
dc.contributor.author	Ben-Dov, E	en_US
dc.contributor.author	Sivan, A	en_US
dc.contributor.author	Kushmaro, A https://orcid.org/0000-0002-1970-9656	en_US
dc.date.issued	2008-11-01	en_US
dc.identifier.citation	Environmental Microbiology, 2008, 10 (11), pp. 2979 - 2990	en_US
dc.identifier.issn	1462-2912	en_US
dc.identifier.uri	http://hdl.handle.net/10453/43888
dc.description.abstract	Diversity, distribution and genetic comparison of Archaea associated with the surface mucus of corals from three genera, namely Acanthastrea sp., Favia sp. and Fungia sp., from the Gulf of Eilat, Israel and from Heron Island, Australia were studied. Sequencing of the 16S rRNA gene of the coral-associated Archaea revealed dominance of Crenarchaeota (79%, on average). In this phylum, 87% of the sequences were similar (≥ 97%) to the Thermoprotei, with 76% of these being similar (≥ 97%) to the ammonium oxidizer, Nitrosopumilus maritimus. Most of the coral-associated euryarchaeotal sequences (69%) were related to marine group II, while other euryarchaeotal clades were found to be related to anaerobic methanotrophs (8%), anaerobic nitrate reducers (i.e. denitrification, 15%) and marine group III (8%). Most of the crenarchaeotal and euryarchaeotal coral-associated 16S rRNA gene sequences from Heron Island (61%) and from the Gulf of Eilat (71%) were closely related (≥ 97%) to sequences previously derived from corals from the Virgin Islands. Analysis of archaeal amoA sequences obtained from the fungiid coral, Fungia granulosa, divided into three clades, all related to archaeal sequences previously obtained from the marine environment. These sequences were distantly related to amoA sequences previously found in association with other coral species. Preliminary experiments suggest that there is active oxidation of ammonia to nitrite in the mucus of F. granulosa. Thus, coral-associated Archaea may contribute to nitrogen recycling in the holobiont, presumably by acting as a nutritional sink for excess ammonium trapped in the mucus layer, through nitrification and denitrification processes. © 2008 The Authors.	en_US
dc.relation.ispartof	Environmental Microbiology	en_US
dc.relation.isbasedon	10.1111/j.1462-2920.2008.01718.x	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Anthozoa	en_US
dc.subject.mesh	Archaea	en_US
dc.subject.mesh	Nitrogen	en_US
dc.subject.mesh	DNA, Archaeal	en_US
dc.subject.mesh	DNA, Ribosomal	en_US
dc.subject.mesh	RNA, Archaeal	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Biodiversity	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Israel	en_US
dc.subject.mesh	Australia	en_US
dc.title	Global distribution and diversity of coral-associated Archaea and their possible role in the coral holobiont nitrogen cycle	en_US
dc.type	Journal Article
utslib.citation.volume	11	en_US
utslib.citation.volume	10	en_US
utslib.for	0603 Evolutionary Biology	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	11	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

Diversity, distribution and genetic comparison of Archaea associated with the surface mucus of corals from three genera, namely Acanthastrea sp., Favia sp. and Fungia sp., from the Gulf of Eilat, Israel and from Heron Island, Australia were studied. Sequencing of the 16S rRNA gene of the coral-associated Archaea revealed dominance of Crenarchaeota (79%, on average). In this phylum, 87% of the sequences were similar (≥ 97%) to the Thermoprotei, with 76% of these being similar (≥ 97%) to the ammonium oxidizer, Nitrosopumilus maritimus. Most of the coral-associated euryarchaeotal sequences (69%) were related to marine group II, while other euryarchaeotal clades were found to be related to anaerobic methanotrophs (8%), anaerobic nitrate reducers (i.e. denitrification, 15%) and marine group III (8%). Most of the crenarchaeotal and euryarchaeotal coral-associated 16S rRNA gene sequences from Heron Island (61%) and from the Gulf of Eilat (71%) were closely related (≥ 97%) to sequences previously derived from corals from the Virgin Islands. Analysis of archaeal amoA sequences obtained from the fungiid coral, Fungia granulosa, divided into three clades, all related to archaeal sequences previously obtained from the marine environment. These sequences were distantly related to amoA sequences previously found in association with other coral species. Preliminary experiments suggest that there is active oxidation of ammonia to nitrite in the mucus of F. granulosa. Thus, coral-associated Archaea may contribute to nitrogen recycling in the holobiont, presumably by acting as a nutritional sink for excess ammonium trapped in the mucus layer, through nitrification and denitrification processes. © 2008 The Authors.

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http://hdl.handle.net/10453/43888