Autofluorescence of Toxoplasma gondii and Neospora caninum cysts in vitro

Lei, Y; Davey, M; Ellis, J

Autofluorescence of Toxoplasma gondii and Neospora caninum cysts in vitro

Lei, Y Davey, M Ellis, J

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Publication Type:: Journal Article
Citation:: Journal of Parasitology, 2005, 91 (1), pp. 17 - 23
Issue Date:: 2005-02-01

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Field	Value	Language
dc.contributor.author	Lei, Y	en_US
dc.contributor.author	Davey, M	en_US
dc.contributor.author	Ellis, J https://orcid.org/0000-0001-7328-4831	en_US
dc.date.issued	2005-02-01	en_US
dc.identifier.citation	Journal of Parasitology, 2005, 91 (1), pp. 17 - 23	en_US
dc.identifier.issn	0022-3395	en_US
dc.identifier.uri	http://hdl.handle.net/10453/4540
dc.description.abstract	Autofluorescence of Toxoplasma gondii and Neospora caninum was studied by fluorescence microscopy during their differentiation from tachyzoites to bradyzoites in vitro using Vero as host cells. Stage conversion into bradyzoites and cysts was confirmed by immunofluorescent microscopy and Western blot analysis using SAGl- and BAGl-specific antibody, respectively. From day 4 postinfection (PI), pale blue autofluorescence of the bradyzoites and tissue cysts was observed with UV light at 330-385 nm, which coincided with the onset of cyst development. This autofluorescence under UV light of bradyzoites and tissue cysts increased in intensity from days 8 to 10 PI. In contrast to the autofluorescence shown by bradyzoites and cysts, tachyzoites and parasitophorous vacuoles containing tachyzoites never autofluoresced at any time examined. Autofluorescence of the cystic stages was of sufficient intensity and duration to allow the detection of cysts and bradyzoites of T. gondii and N. caninum. In this study, we describe for the first time the autofluorescence properties of in vitro-induced bradyzoites and cysts of T. gondii and N. caninum. © American Society of Parasitologists 2005.	en_US
dc.relation.ispartof	Journal of Parasitology	en_US
dc.relation.isbasedon	10.1645/GE-355R	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Vero Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Cercopithecus aethiops	en_US
dc.subject.mesh	Neospora	en_US
dc.subject.mesh	Toxoplasma	en_US
dc.subject.mesh	Heat-Shock Proteins	en_US
dc.subject.mesh	Protozoan Proteins	en_US
dc.subject.mesh	Antibodies, Monoclonal	en_US
dc.subject.mesh	Antibodies, Protozoan	en_US
dc.subject.mesh	Antigens, Protozoan	en_US
dc.subject.mesh	Antigens, Surface	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Blotting, Western	en_US
dc.subject.mesh	Serial Passage	en_US
dc.subject.mesh	Chlorocebus aethiops	en_US
dc.title	Autofluorescence of Toxoplasma gondii and Neospora caninum cysts in vitro	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	91	en_US
utslib.for	0608 Zoology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	91	en_US

Abstract:

Autofluorescence of Toxoplasma gondii and Neospora caninum was studied by fluorescence microscopy during their differentiation from tachyzoites to bradyzoites in vitro using Vero as host cells. Stage conversion into bradyzoites and cysts was confirmed by immunofluorescent microscopy and Western blot analysis using SAGl- and BAGl-specific antibody, respectively. From day 4 postinfection (PI), pale blue autofluorescence of the bradyzoites and tissue cysts was observed with UV light at 330-385 nm, which coincided with the onset of cyst development. This autofluorescence under UV light of bradyzoites and tissue cysts increased in intensity from days 8 to 10 PI. In contrast to the autofluorescence shown by bradyzoites and cysts, tachyzoites and parasitophorous vacuoles containing tachyzoites never autofluoresced at any time examined. Autofluorescence of the cystic stages was of sufficient intensity and duration to allow the detection of cysts and bradyzoites of T. gondii and N. caninum. In this study, we describe for the first time the autofluorescence properties of in vitro-induced bradyzoites and cysts of T. gondii and N. caninum. © American Society of Parasitologists 2005.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/4540