Drug resistance does not correlate with resistance to Fas-mediated apoptosis.

Cullen, KV; Davey, RA; Davey, MW

Drug resistance does not correlate with resistance to Fas-mediated apoptosis.

Cullen, KV Davey, RA Davey, MW

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Publication Type:: Journal Article
Citation:: Leuk Res, 2001, 25 (1), pp. 69 - 75
Issue Date:: 2001-01

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Field	Value	Language
dc.contributor.author	Cullen, KV	en_US
dc.contributor.author	Davey, RA	en_US
dc.contributor.author	Davey, MW	en_US
dc.date.issued	2001-01	en_US
dc.identifier.citation	Leuk Res, 2001, 25 (1), pp. 69 - 75	en_US
dc.identifier.issn	0145-2126	en_US
dc.identifier.uri	http://hdl.handle.net/10453/4788
dc.description.abstract	Recent reports have correlated multidrug resistance (MDR) and P-glycoprotein expression with decreased Fas expression and resistance to Fas-mediated apoptosis. We report the MRP-overexpressing MDR subline CEM/E1000 has the same Fas expression (MFI 74.3 +/- 0.7) as the parental CCRF-CEM T-cell leukaemia cells (MFI 70.0 +/- 3.6; P>0.05), and that the level of apoptosis induced by anti-Fas antibody or drug was similar in both cell lines. Further the P-glycoprotein-expressing CEM/VLB(100) subline of the CCRF-CEM cells showed increased Fas expression (MFI 114.8 +/- 3.6; P<0.001) and no resistance to Fas-mediated apoptosis. This questions the hypothesis that selection of drug resistance results in resistance to Fas-mediated apoptosis, with important implications for the rational use of immunotherapy in the treatment of drug resistant cancer.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Leuk Res	en_US
dc.relation.isbasedon	10.1016/s0145-2126(00)00085-0	en_US
dc.subject.classification	Immunology	en_US
dc.subject.mesh	Tumor Cells, Cultured	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Leukemia, T-Cell	en_US
dc.subject.mesh	Cisplatin	en_US
dc.subject.mesh	Etoposide	en_US
dc.subject.mesh	Immunoglobulin M	en_US
dc.subject.mesh	ATP-Binding Cassette Transporters	en_US
dc.subject.mesh	Multidrug Resistance-Associated Proteins	en_US
dc.subject.mesh	Antineoplastic Agents	en_US
dc.subject.mesh	Antineoplastic Agents, Phytogenic	en_US
dc.subject.mesh	Antibodies, Monoclonal	en_US
dc.subject.mesh	Drug Resistance, Multiple	en_US
dc.subject.mesh	Apoptosis	en_US
dc.subject.mesh	Drug Resistance, Neoplasm	en_US
dc.subject.mesh	fas Receptor	en_US
dc.subject.mesh	ATP Binding Cassette Transporter, Subfamily B, Member 1	en_US
dc.title	Drug resistance does not correlate with resistance to Fas-mediated apoptosis.	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	25	en_US
utslib.location.activity	England	en_US
utslib.for	070708 Veterinary Parasitology	en_US
utslib.for	100302 Bioprocessing, Bioproduction and Bioproducts	en_US
utslib.for	060199 Biochemistry and Cell Biology not elsewhere classified	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	25	en_US

Abstract:

Recent reports have correlated multidrug resistance (MDR) and P-glycoprotein expression with decreased Fas expression and resistance to Fas-mediated apoptosis. We report the MRP-overexpressing MDR subline CEM/E1000 has the same Fas expression (MFI 74.3 +/- 0.7) as the parental CCRF-CEM T-cell leukaemia cells (MFI 70.0 +/- 3.6; P>0.05), and that the level of apoptosis induced by anti-Fas antibody or drug was similar in both cell lines. Further the P-glycoprotein-expressing CEM/VLB(100) subline of the CCRF-CEM cells showed increased Fas expression (MFI 114.8 +/- 3.6; P<0.001) and no resistance to Fas-mediated apoptosis. This questions the hypothesis that selection of drug resistance results in resistance to Fas-mediated apoptosis, with important implications for the rational use of immunotherapy in the treatment of drug resistant cancer.

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http://hdl.handle.net/10453/4788