Comparative analysis of quantitative methodologies for Vibrionaceae biofilms

Chavez-Dozal, AA; Nourabadi, N; Erken, M; McDougald, D; Nishiguchi, MK

Comparative analysis of quantitative methodologies for Vibrionaceae biofilms

Chavez-Dozal, AA Nourabadi, N Erken, M McDougald, D

Nishiguchi, MK

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Publication Type:: Journal Article
Citation:: Folia Microbiologica, 2016, 61 (6), pp. 449 - 453
Issue Date:: 2016-11-01

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Field	Value	Language
dc.contributor.author	Chavez-Dozal, AA	en_US
dc.contributor.author	Nourabadi, N	en_US
dc.contributor.author	Erken, M	en_US
dc.contributor.author	McDougald, D https://orcid.org/0000-0001-5827-8441	en_US
dc.contributor.author	Nishiguchi, MK	en_US
dc.date.available	2016-03-09	en_US
dc.date.issued	2016-11-01	en_US
dc.identifier.citation	Folia Microbiologica, 2016, 61 (6), pp. 449 - 453	en_US
dc.identifier.issn	0015-5632	en_US
dc.identifier.uri	http://hdl.handle.net/10453/49140
dc.description.abstract	© 2016, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. Multiple symbiotic and free-living Vibrio spp. grow as a form of microbial community known as a biofilm. In the laboratory, methods to quantify Vibrio biofilm mass include crystal violet staining, direct colony-forming unit (CFU) counting, dry biofilm cell mass measurement, and observation of development of wrinkled colonies. Another approach for bacterial biofilms also involves the use of tetrazolium (XTT) assays (used widely in studies of fungi) that are an appropriate measure of metabolic activity and vitality of cells within the biofilm matrix. This study systematically tested five techniques, among which the XTT assay and wrinkled colony measurement provided the most reproducible, accurate, and efficient methods for the quantitative estimation of Vibrionaceae biofilms.	en_US
dc.relation.ispartof	Folia Microbiologica	en_US
dc.relation.isbasedon	10.1007/s12223-016-0456-9	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Vibrionaceae	en_US
dc.subject.mesh	Bacteriological Techniques	en_US
dc.title	Comparative analysis of quantitative methodologies for Vibrionaceae biofilms	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	61	en_US
utslib.for	1108 Medical Microbiology	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	61	en_US

Abstract:

© 2016, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. Multiple symbiotic and free-living Vibrio spp. grow as a form of microbial community known as a biofilm. In the laboratory, methods to quantify Vibrio biofilm mass include crystal violet staining, direct colony-forming unit (CFU) counting, dry biofilm cell mass measurement, and observation of development of wrinkled colonies. Another approach for bacterial biofilms also involves the use of tetrazolium (XTT) assays (used widely in studies of fungi) that are an appropriate measure of metabolic activity and vitality of cells within the biofilm matrix. This study systematically tested five techniques, among which the XTT assay and wrinkled colony measurement provided the most reproducible, accurate, and efficient methods for the quantitative estimation of Vibrionaceae biofilms.

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http://hdl.handle.net/10453/49140