The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132

Remenyi, J; Bajan, S; Fuller-Pace, FV; Arthur, JSC; Hutvagner, G

The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132

Remenyi, J Bajan, S

Fuller-Pace, FV Arthur, JSC Hutvagner, G

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Publication Type:: Journal Article
Citation:: Scientific Reports, 2016, 6
Issue Date:: 2016-03-07

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Field	Value	Language
dc.contributor.author	Remenyi, J	en_US
dc.contributor.author	Bajan, S https://orcid.org/0000-0001-6934-5240	en_US
dc.contributor.author	Fuller-Pace, FV	en_US
dc.contributor.author	Arthur, JSC	en_US
dc.contributor.author	Hutvagner, G https://orcid.org/0000-0002-7231-9446	en_US
dc.date.available	2016-02-23	en_US
dc.date.issued	2016-03-07	en_US
dc.identifier.citation	Scientific Reports, 2016, 6	en_US
dc.identifier.uri	http://hdl.handle.net/10453/50310
dc.description.abstract	miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or inhibit miRNA maturation and can be controlled in a cell, tissue and species-specific manners or in response to environmental stimuli. In this study we dissect the molecular mechanism that promotes the overexpression of miR-132 in mice over its related, co-transcribed and co-regulated miRNA, miR-212. We have shown that the loop structure of miR-132 is a key determinant for its efficient processing in cells. We have also identified a range of RNA binding proteins that recognize the loop of miR-132 and influence both miR-132 and miR-212 processing. The DEAD box helicase p72/DDX17 was identified as a factor that facilitates the specific processing of miR-132.	en_US
dc.relation	http://purl.org/au-research/grants/arc/FT110100455
dc.relation	http://purl.org/au-research/grants/arc/
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/srep22848	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Hela Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	Gene Expression Regulation	en_US
dc.subject.mesh	RNA Processing, Post-Transcriptional	en_US
dc.subject.mesh	Up-Regulation	en_US
dc.subject.mesh	Nucleic Acid Conformation	en_US
dc.subject.mesh	Models, Molecular	en_US
dc.subject.mesh	DEAD-box RNA Helicases	en_US
dc.subject.mesh	HeLa Cells	en_US
dc.title	The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0999 Other Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	6	en_US

Abstract:

miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or inhibit miRNA maturation and can be controlled in a cell, tissue and species-specific manners or in response to environmental stimuli. In this study we dissect the molecular mechanism that promotes the overexpression of miR-132 in mice over its related, co-transcribed and co-regulated miRNA, miR-212. We have shown that the loop structure of miR-132 is a key determinant for its efficient processing in cells. We have also identified a range of RNA binding proteins that recognize the loop of miR-132 and influence both miR-132 and miR-212 processing. The DEAD box helicase p72/DDX17 was identified as a factor that facilitates the specific processing of miR-132.

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http://hdl.handle.net/10453/50310