The miRNA biogenesis factors, p72/DDX17 and KHSRP regulate the protein level of Ago2 in human cells.
- Publication Type:
- Journal Article
- Biochim Biophys Acta, 1859 (10), pp. 1299 - 1305
|dc.identifier.citation||Biochim Biophys Acta, 1859 (10), pp. 1299 - 1305||en_US|
|dc.description.abstract||MicroRNAs (miRNAs) are short (21-23nt long) RNAs that post-transcriptionally regulate gene expression in plants and animals. They are key regulators in all biological processes. In mammalian cells miRNAs are loaded into one of the four members of the Argonaute (Ago) protein family to form the RNA-induced silencing complex (RISC). RISCs inhibit the translation of mRNAs that share sequence complementarity with their loaded miRNAs. miRNA processing and miRNA-mediated gene regulation are highly regulated processes and involve many RNA-binding proteins as auxiliary factors. Here we show that the two RNA-binding proteins, p72 and KHSRP, both with known roles in promoting miRNA biogenesis, regulate the protein level of human Ago2 in transformed human cells. We determined that p72 and KHSRP influence Ago2 stability by regulating miRNA levels in the cell and that loss of p72/KHSRP results in a decrease of unloaded Ago2.||en_US|
|dc.relation.ispartof||Biochim Biophys Acta||en_US|
|dc.subject.mesh||Cell Line, Tumor||en_US|
|dc.subject.mesh||DEAD-box RNA Helicases||en_US|
|dc.subject.mesh||Gene Expression Regulation||en_US|
|dc.subject.mesh||RNA-Induced Silencing Complex||en_US|
|dc.title||The miRNA biogenesis factors, p72/DDX17 and KHSRP regulate the protein level of Ago2 in human cells.||en_US|
|utslib.for||06 Biological Sciences||en_US|
|utslib.for||02 Physical Sciences||en_US|
|pubs.organisational-group||/University of Technology Sydney|
|pubs.organisational-group||/University of Technology Sydney/Faculty of Engineering and Information Technology|
|pubs.organisational-group||/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering|
|pubs.organisational-group||/University of Technology Sydney/Faculty of Science|
|pubs.organisational-group||/University of Technology Sydney/Strength - CHT - Health Technologies|
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