Culture of Neospora caninum in the presence of a Mycoplasma Removal Agent results in the selection of a mutant population of tachyzoites

Hudson, A; Ellis, JT

Culture of Neospora caninum in the presence of a Mycoplasma Removal Agent results in the selection of a mutant population of tachyzoites

Hudson, A Ellis, JT

Permalink

Publication Type:: Journal Article
Citation:: Parasitology, 2005, 130 (6), pp. 607 - 610
Issue Date:: 2005-06-01

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download full textAdobe PDF (72.65 kB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Hudson, A	en_US
dc.contributor.author	Ellis, JT https://orcid.org/0000-0001-7328-4831	en_US
dc.date.issued	2005-06-01	en_US
dc.identifier.citation	Parasitology, 2005, 130 (6), pp. 607 - 610	en_US
dc.identifier.issn	0031-1820	en_US
dc.identifier.uri	http://hdl.handle.net/10453/5883
dc.description.abstract	Mycoplasmas are common contaminants of eukaryotic cells grown in tissue culture. A commercially available Mycoplasma Removal Agent (MRA) was therefore assessed for its effect on tachyzoites of Neospora caninum, in order to determine its suitability for further use in parasite cell cultures. Analyses of tachyzoite and excreted-secreted proteins and antigens by SDS-PAGE and Western blotting show that MRA treatment results in the rapid selection of a mutant population that differs from the control and parental lines in its protein and antigen content. The treatment of N. caninum cultures with MRA is therefore not recommended for the eradication of Mycoplasma. © 2005 Cambridge University Press.	en_US
dc.relation.ispartof	Parasitology	en_US
dc.relation.isbasedon	10.1017/S0031182004007115	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Neospora	en_US
dc.subject.mesh	Mycoplasma	en_US
dc.subject.mesh	Quinolones	en_US
dc.subject.mesh	Protozoan Proteins	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Cell Culture Techniques	en_US
dc.subject.mesh	Gene Expression	en_US
dc.subject.mesh	Mutation	en_US
dc.title	Culture of Neospora caninum in the presence of a Mycoplasma Removal Agent results in the selection of a mutant population of tachyzoites	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	130	en_US
utslib.for	070708 Veterinary Parasitology	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	130	en_US

Abstract:

Mycoplasmas are common contaminants of eukaryotic cells grown in tissue culture. A commercially available Mycoplasma Removal Agent (MRA) was therefore assessed for its effect on tachyzoites of Neospora caninum, in order to determine its suitability for further use in parasite cell cultures. Analyses of tachyzoite and excreted-secreted proteins and antigens by SDS-PAGE and Western blotting show that MRA treatment results in the rapid selection of a mutant population that differs from the control and parental lines in its protein and antigen content. The treatment of N. caninum cultures with MRA is therefore not recommended for the eradication of Mycoplasma. © 2005 Cambridge University Press.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/5883