Study of the selectivity of reversed-phase columns for the separation of polycarboxylic acids and polyphenol compounds.

Whelan, TJ; Gray, MJ; Slonecker, PJ; Shalliker, RA; Wilson, MA

Study of the selectivity of reversed-phase columns for the separation of polycarboxylic acids and polyphenol compounds.

Whelan, TJ Gray, MJ Slonecker, PJ Shalliker, RA Wilson, MA

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Publication Type:: Journal Article
Citation:: J Chromatogr A, 2005, 1097 (1-2), pp. 148 - 156
Issue Date:: 2005-12-02

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Field	Value	Language
dc.contributor.author	Whelan, TJ	en_US
dc.contributor.author	Gray, MJ	en_US
dc.contributor.author	Slonecker, PJ	en_US
dc.contributor.author	Shalliker, RA	en_US
dc.contributor.author	Wilson, MA	en_US
dc.date.available	2005-08-10	en_US
dc.date.issued	2005-12-02	en_US
dc.identifier.citation	J Chromatogr A, 2005, 1097 (1-2), pp. 148 - 156	en_US
dc.identifier.issn	0021-9673	en_US
dc.identifier.uri	http://hdl.handle.net/10453/618
dc.description.abstract	A systematic investigation was undertaken into the relative separation performance of five reversed-phase chromatography columns including some commercially new hybrid packed columns for a series of polycarboxylic acids and polyphenol compounds. Information theory (IT) and factor analysis (FA), together with a basic evaluation of retention information (band shape, retention factor and elution order) were used to compare four columns to a conventional C18 column. The results revealed very little difference in retention behaviour between the Phenomenex Aqua C18 column, the Waters XTerra RP C18 column, and the conventional Phenomenex Luna C18 column. However, there were notable differences in the retention processes between the Phenomenex Synergi polar-RP column, which is an ether-linked phenyl base with polar endcapping, and the Luna C18 column. The most significant differences were observed between the Luna C18 column and a Phenomenex Luna Cyano column. However, the limited degree of retention of the polycarboxylic acids and polyphenol compounds on the Luna Cyano column permits only limited use for the separation of these types of compounds. Overall, the Phenomenex Synergi polar-RP column exhibited the best performance for the separation of the test solutes compared to that of the conventional C18 column, with IT yielding an Informational Similarity of 0.99 and FA a moderate correlation coefficient of 0.70. The Phenomenex Synergi polar-RP column gave the best peak shape and offered substantial selectivity differences thereby providing a good alternative over the conventional C18 column for separating polycarboxylic acids and polyphenols.	en_US
dc.language	eng	en_US
dc.relation.ispartof	J Chromatogr A	en_US
dc.relation.isbasedon	10.1016/j.chroma.2005.08.026	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Silicon Dioxide	en_US
dc.subject.mesh	Carboxylic Acids	en_US
dc.subject.mesh	Phenols	en_US
dc.subject.mesh	Flavonoids	en_US
dc.subject.mesh	Polymers	en_US
dc.subject.mesh	Chromatography, Liquid	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Kinetics	en_US
dc.subject.mesh	Adsorption	en_US
dc.subject.mesh	Particle Size	en_US
dc.subject.mesh	Algorithms	en_US
dc.subject.mesh	Polyphenols	en_US
dc.title	Study of the selectivity of reversed-phase columns for the separation of polycarboxylic acids and polyphenol compounds.	en_US
dc.type	Journal Article
utslib.citation.volume	1-2	en_US
utslib.citation.volume	1097	en_US
utslib.location.activity	Netherlands	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	09 Engineering	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Design, Architecture and Building
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	1-2	en_US
pubs.publication-status	Published	en_US
pubs.volume	1097	en_US

Abstract:

A systematic investigation was undertaken into the relative separation performance of five reversed-phase chromatography columns including some commercially new hybrid packed columns for a series of polycarboxylic acids and polyphenol compounds. Information theory (IT) and factor analysis (FA), together with a basic evaluation of retention information (band shape, retention factor and elution order) were used to compare four columns to a conventional C18 column. The results revealed very little difference in retention behaviour between the Phenomenex Aqua C18 column, the Waters XTerra RP C18 column, and the conventional Phenomenex Luna C18 column. However, there were notable differences in the retention processes between the Phenomenex Synergi polar-RP column, which is an ether-linked phenyl base with polar endcapping, and the Luna C18 column. The most significant differences were observed between the Luna C18 column and a Phenomenex Luna Cyano column. However, the limited degree of retention of the polycarboxylic acids and polyphenol compounds on the Luna Cyano column permits only limited use for the separation of these types of compounds. Overall, the Phenomenex Synergi polar-RP column exhibited the best performance for the separation of the test solutes compared to that of the conventional C18 column, with IT yielding an Informational Similarity of 0.99 and FA a moderate correlation coefficient of 0.70. The Phenomenex Synergi polar-RP column gave the best peak shape and offered substantial selectivity differences thereby providing a good alternative over the conventional C18 column for separating polycarboxylic acids and polyphenols.

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