A genomic island in Vibrio cholerae with VPI-1 site-specific recombination characteristics contains CRISPR-Cas and type VI secretion modules

Labbate, M; Orata, FD; Petty, NK; Jayatilleke, ND; King, WL; Kirchberger, PC; Allen, C; Mann, G; Mutreja, A; Thomson, NR; Boucher, Y; Charles, IG

A genomic island in Vibrio cholerae with VPI-1 site-specific recombination characteristics contains CRISPR-Cas and type VI secretion modules

Labbate, M

Orata, FD Petty, NK

Jayatilleke, ND King, WL

Kirchberger, PC Allen, C Mann, G Mutreja, A Thomson, NR Boucher, Y Charles, IG

Permalink

Publication Type:: Journal Article
Citation:: Scientific Reports, 2016, 6
Issue Date:: 2016-11-15

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Download Accepted Manuscript VersionAdobe PDF (936.49 kB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Labbate, M https://orcid.org/0000-0003-1428-3382	en_US
dc.contributor.author	Orata, FD	en_US
dc.contributor.author	Petty, NK https://orcid.org/0000-0001-6528-9886	en_US
dc.contributor.author	Jayatilleke, ND	en_US
dc.contributor.author	King, WL https://orcid.org/0000-0001-7272-8242	en_US
dc.contributor.author	Kirchberger, PC	en_US
dc.contributor.author	Allen, C	en_US
dc.contributor.author	Mann, G	en_US
dc.contributor.author	Mutreja, A	en_US
dc.contributor.author	Thomson, NR	en_US
dc.contributor.author	Boucher, Y	en_US
dc.contributor.author	Charles, IG https://orcid.org/0000-0002-2423-1167	en_US
dc.date.available	2016-10-10	en_US
dc.date.issued	2016-11-15	en_US
dc.identifier.citation	Scientific Reports, 2016, 6	en_US
dc.identifier.uri	http://hdl.handle.net/10453/66517
dc.description.abstract	© 2016 The Author(s). Cholera is a devastating diarrhoeal disease caused by certain strains of serogroup O1/O139 Vibrio cholerae. Mobile genetic elements such as genomic islands (GIs) have been pivotal in the evolution of O1/O139 V. cholerae. Perhaps the most important GI involved in cholera disease is the V. cholerae pathogenicity island 1 (VPI-1). This GI contains the toxin-coregulated pilus (TCP) gene cluster that is necessary for colonization of the human intestine as well as being the receptor for infection by the cholera-toxin bearing CTX phage. In this study, we report a GI (designated GIVchS12) from a non-O1/O139 strain of V. cholerae that is present in the same chromosomal location as VPI-1, contains an integrase gene with 94% nucleotide and 100% protein identity to the VPI-1 integrase, and attachment (att) sites 100% identical to those found in VPI-1. However, instead of TCP and the other accessory genes present in VPI-1, GIVchS12 contains a CRISPR-Cas element and a type VI secretion system (T6SS). GIs similar to GIVchS12 were identified in other V. cholerae genomes, also containing CRISPR-Cas elements and/or T6SS's. This study highlights the diversity of GIs circulating in natural V. cholerae populations and identifies GIs with VPI-1 recombination characteristics as a propagator of CRISPR-Cas and T6SS modules.	en_US
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/srep36891	en_US
dc.subject.mesh	Vibrio cholerae non-O1	en_US
dc.subject.mesh	Vibrio cholerae O139	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Virulence Factors	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Genomic Islands	en_US
dc.subject.mesh	Multigene Family	en_US
dc.subject.mesh	Clustered Regularly Interspaced Short Palindromic Repeats	en_US
dc.subject.mesh	Type VI Secretion Systems	en_US
dc.title	A genomic island in Vibrio cholerae with VPI-1 site-specific recombination characteristics contains CRISPR-Cas and type VI secretion modules	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.for	110309 Infectious Diseases	en_US
utslib.for	110801 Medical Bacteriology	en_US
utslib.for	060501 Bacteriology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	6	en_US

Abstract:

© 2016 The Author(s). Cholera is a devastating diarrhoeal disease caused by certain strains of serogroup O1/O139 Vibrio cholerae. Mobile genetic elements such as genomic islands (GIs) have been pivotal in the evolution of O1/O139 V. cholerae. Perhaps the most important GI involved in cholera disease is the V. cholerae pathogenicity island 1 (VPI-1). This GI contains the toxin-coregulated pilus (TCP) gene cluster that is necessary for colonization of the human intestine as well as being the receptor for infection by the cholera-toxin bearing CTX phage. In this study, we report a GI (designated GIVchS12) from a non-O1/O139 strain of V. cholerae that is present in the same chromosomal location as VPI-1, contains an integrase gene with 94% nucleotide and 100% protein identity to the VPI-1 integrase, and attachment (att) sites 100% identical to those found in VPI-1. However, instead of TCP and the other accessory genes present in VPI-1, GIVchS12 contains a CRISPR-Cas element and a type VI secretion system (T6SS). GIs similar to GIVchS12 were identified in other V. cholerae genomes, also containing CRISPR-Cas elements and/or T6SS's. This study highlights the diversity of GIs circulating in natural V. cholerae populations and identifies GIs with VPI-1 recombination characteristics as a propagator of CRISPR-Cas and T6SS modules.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/66517